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Aspergillus oryzae LC-MSMS - Comparative proteomic analysis: SclR is importantly involved in carbohydrate metabolism in Aspergillus oryzae

ABSTRACT: We previously identified the bHLH transcription factor SclR and found that the loss of SclR function led to significant phenotypic changes, such as rapid protein degradation and cell lysis in dextrin-polypeptone-yeast extract liquid medium. Therefore, this study presents a comparative assessment at the proteome level of the intracellular differences between an sclR-disrupted strain and a control strain using isobaric tandem mass tag (TMT) labeling for quantification.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Aspergillus Oryzae Rib40

SUBMITTER: Feng-Jie Jin

LAB HEAD: Feng-Jie Jin

PROVIDER: PXD007829 | Pride | 2017-11-07

REPOSITORIES: Pride

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Comparative proteomic analysis: SclR is importantly involved in carbohydrate metabolism in Aspergillus oryzae.

Jin Feng-Jie FJ Han Pei P Zhuang Miao M Zhang Zhi-Min ZM Jin Long L Koyama Yasuji Y

Applied microbiology and biotechnology 20171102 1

The helix-loop-helix (HLH) family of transcriptional factors is a key player in a wide range of developmental processes in organisms from mammals to microbes. We previously identified the bHLH transcription factor SclR in Aspergillus oryzae and found that the loss of SclR function led to significant phenotypic changes, such as rapid protein degradation and cell lysis in dextrin-polypeptone-yeast extract liquid medium. The result implied that SclR is potentially important in both traditional ferm ...[more]

PMID: 29098410

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