Dataset Information

Mass spectrometry of excretory/secretory products (ectosomes, exosomes, and soluble proteins) secreted by Toxoplasma gondii tachyzoites isolated from an infected murine model.

ABSTRACT: Toxoplasma gondii is an intracellular parasite that infects all nucleated cells; to date, this organism is considered as one of the most successful parasites in the world. During infection, internalization, and proliferation the parasite excretes/secretes a high number of proteins, several of them with identified roles, including MIC proteins, ROP proteins and GRA proteins involved in adhesion, invasion and intracellular proliferation respectively. The characterization of the secretome of Toxoplasma gondii opens the possibility to find new molecules for the design of future anti-parasitic strategies. The complete secretome and proteome of the parasite isolated from the murine model as well as their quantification by MS analysis are now available in the present report.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Toxoplasma Gondii Gt1

TISSUE(S): Excretion, Secretion

DISEASE(S): Toxoplasmosis

SUBMITTER: Emmanuel Rios-Castro

LAB HEAD: Ricardo Mondragón-Flores

PROVIDER: PXD013767 | Pride | 2019-08-29

REPOSITORIES: Pride

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Publications

Proteomic and structural characterization of self-assembled vesicles from excretion/secretion products of Toxoplasma gondii.

Ramírez-Flores Carlos J CJ Cruz-Mirón Rosalba R Mondragón-Castelán Mónica E ME González-Pozos Sirenia S Ríos-Castro Emmanuel E Mondragón-Flores Ricardo R

Journal of proteomics 20190818

After the cell invasion, the parasite Toxoplasma gondii locates within a parasitophorous vacuole to proliferate. It continuously modifies the composition of the parasitophorous vacuole by the secretion of GRA and ROP proteins, some of which become inserted into the vacuole membrane, remain as soluble proteins or involved in the intravacuolar network. In this report, we analyze the excretion/secretion products and the vesicles released by extracellular tachyzoites, this structures were morphologi ...[more]

PMID: 31434009

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Project description:Toxoplasma gondii is a globally distributed obligate intracellular parasite which can cause zoonotic toxoplasmosis with great harms. The average death time of mice that infected with Toxoplasma gondii RH strain tachyzoites recovered from the liquid nitrogen was shortened after multiple generations. It has been reported that the parasite is in a state of static virulence during cryopreservation and the virulence of the protozoan parasite can be enhanced after continuous passages in hosts under laboratory conditions. However, no research has been conducted to elucidate its biological mechanism. Herein, we sequenced the T. gondii transcriptome using RNA-Seq technology and performed de novo assembly to investigated the virulence factors expression changes by comparing gene expression profiles between incipiently recovered and completely resuscitated tachyzoites. Transcriptome analysis identified 1,951 differentially expressed transcripts in infected liver, of which 1,752 were significantly downregulated and 199 upregulated. We identified many differentially expressed proteins and genes, including serine/threonine kinase, calnexin, myosin and microtubule-associated protein which have previously been reported to be either involved in cell adhesion, parasite gliding or participate in cell invasion. The great majority of the virulence factors including microneme proteins, rhoptry proteins and dense granule proteins were upregulated in fully recovered tachyzoites. The enhanced virulence of recovered Toxoplasma gondii RH strain from the liquid nitrogen is associated with the up-regulated expression of MICs, ROPs and GRAs. Our data will facilitate future genomic research and in-depth annotation of Toxoplasma gondii RH strain genomes. This study provides a profile of the candidate genes that are suspected to be involved with virulence enhancement of recovered Toxoplasma gondii RH strain tachyzoites. Many further studies should be carried out to confirm the function of the candidate genes. Moreover, the preliminary identification of genes and pathways exhibiting differential expression in complete resuscitation stage may further our general understanding of virulence enhancement in this parasite.

Dataset Information

Mass spectrometry of excretory/secretory products (ectosomes, exosomes, and soluble proteins) secreted by Toxoplasma gondii tachyzoites isolated from an infected murine model.

Publications

Proteomic and structural characterization of self-assembled vesicles from excretion/secretion products of Toxoplasma gondii.

Similar Datasets

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