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Quantitative LC-MS/MS Proteomics of human brain microvessels

ABSTRACT: We report label-free quantification of xenobiotic metabolizing enzymes (XME), transporters, redox enzymes, proteases, nucleases and tight junction proteins in 22 human brain microvessel samples. More than 3500 proteins were identified and quantified. These data can be used in physiologically based pharmacokinetic models to predict drug disposition in the brain and permitting dose adjustment (precision dosing) in special populations of patients, such as those with dementia.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

SUBMITTER: Zubida Al-Majdoub

LAB HEAD: Jill Barber

PROVIDER: PXD021018 | Pride | 2021-09-09

REPOSITORIES: Pride

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Mass spectrometry-based abundance atlas of ABC transporters in human liver, gut, kidney, brain and skin.

Al-Majdoub Zubida M ZM Achour Brahim B Couto Narciso N Howard Martyn M Elmorsi Yasmine Y Scotcher Daniel D Alrubia Sarah S El-Khateeb Eman E Vasilogianni Areti-Maria AM Alohali Noura N Neuhoff Sibylle S Schmitt Lutz L Rostami-Hodjegan Amin A Barber Jill J

FEBS letters 20201203 23

ABC transporters (ATP-binding cassette transporter) traffic drugs and their metabolites across membranes, making ABC transporter expression levels a key factor regulating local drug concentrations in different tissues and individuals. Yet, quantification of ABC transporters remains challenging because they are large and low-abundance transmembrane proteins. Here, we analysed 200 samples of crude and membrane-enriched fractions from human liver, kidney, intestine, brain microvessels and skin, by ...[more]

PMID: 33128234

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