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The modulation of CHD4 nucleosome remodelling activity by auxiliary domains suggests unifies mechanistic aspects of chromatin remodelling across enzyme families

ABSTRACT: We determine the impact of auxiliary domains on the nucleosome sliding activity of CHD4. This dataset comprises the crosslinking mass spectrometry data we reported.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Jason Low

LAB HEAD: Joel Mackay

PROVIDER: PXD033633 | Pride | 2023-03-11

REPOSITORIES: Pride

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The role of auxiliary domains in modulating CHD4 activity suggests mechanistic commonality between enzyme families.

Zhong Yichen Y Moghaddas Sani Hakimeh H Paudel Bishnu P BP Low Jason K K JKK Silva Ana P G APG Mueller Stefan S Deshpande Chandrika C Panjikar Santosh S Reid Xavier J XJ Bedward Max J MJ van Oijen Antoine M AM Mackay Joel P JP

Nature communications 20221206 1

CHD4 is an essential, widely conserved ATP-dependent translocase that is also a broad tumour dependency. In common with other SF2-family chromatin remodelling enzymes, it alters chromatin accessibility by repositioning histone octamers. Besides the helicase and adjacent tandem chromodomains and PHD domains, CHD4 features 1000 residues of N- and C-terminal sequence with unknown structure and function. We demonstrate that these regions regulate CHD4 activity through different mechanisms. An N-term ...[more]

PMID: 36473839

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Expression data from glioblastoma cells after ZFHX4 or CHD4 suppression.

Project description:ZFHX4 and CHD4 suppression independently shift tumor initiating cells out of a stem like state and toward a differentiated morphology. After gene suppression via transduction of a lentivirally mediated shRNA construct, RNA was extracted 3 and 5 days later and was hybridized on Affymetrix microarrays. Total RNA was extracted 3 and 5 days after shRNA transduction of 0308 TICs, for 3 (for CHD4) or 5 (ZFHX4) independent experiments. Complementary RNA synthesis and hybridization/scanning of U133 plus 2.0 microarrays using GeneChip products (Affymetrix) was done as described in the GeneChip manual.

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