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Arterial-Venous Endothelial Cell Cycle Proteins Differential Expression

ABSTRACT: HUVEC-FUCCI cells were used to demonstrate that different endothelial cell cycle states provide distict windows of opportunity for gene expression in response to extrinsic signals. HUVEC-FUCCI were FACS-isolated into three different cell cycle states. Peptide digests from the resulting lysates showed differentially expressed proteins among the three cell cycles. These studies show that endothelial cell cycle state determines the propensity for arterial vs. venous fate specification.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Endothelial Cell Of Umbilical Vein, Cell Culture

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Erin Jeffery

LAB HEAD: Dr. Gloria Sheynkman

PROVIDER: PXD036326 | Pride | 2022-10-12

REPOSITORIES: Pride

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Endothelial cell cycle state determines propensity for arterial-venous fate.

Chavkin Nicholas W NW Genet Gael G Poulet Mathilde M Jeffery Erin D ED Marziano Corina C Genet Nafiisha N Vasavada Hema H Nelson Elizabeth A EA Acharya Bipul R BR Kour Anupreet A Aragon Jordon J McDonnell Stephanie P SP Huba Mahalia M Sheynkman Gloria M GM Walsh Kenneth K Hirschi Karen K KK

Nature communications 20221006 1

During blood vessel development, endothelial cells become specified toward arterial or venous fates to generate a circulatory network that provides nutrients and oxygen to, and removes metabolic waste from, all tissues. Arterial-venous specification occurs in conjunction with suppression of endothelial cell cycle progression; however, the mechanistic role of cell cycle state is unknown. Herein, using Cdh5-CreER<sup>T2</sup>;R26FUCCI2aR reporter mice, we find that venous endothelial cells are enr ...[more]

PMID: 36202789

Publication: 1/2

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