Proteomics

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Loss of Rnd3/RhoE induces entosis in human hepatocellular carcinoma

ABSTRACT: Entosis is a process that leads to the formation of cell-in-cell structures commonly found in cancers. Here, we identified entosis in hepatocellular carcinoma and the loss of Rnd3 as an efficient inducer of this mechanism. We characterized the different stages and the molecular regulators of entosis induced after silencing of Rnd3. We demonstrated that this process is dependent on RhoA/ROCK pathway, but independent on E-cadherin. The proteomic analysis of entotic cells allowed us to identify LAMP-1 as a protein implicated not only in the degradation final stage of entosis, but also in the full mechanism. By analyzing entosis in human hepatocellular carcinoma samples, we found a relationship between the presence of entotic cells and the metastatic potential of tumors. Altogether, these data suggest the involvement of entosis in liver tumor progression and highlight a new perspective for the entosis analysis in medicine research as a novel therapeutic target.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Dupuy Jean-William  

LAB HEAD: Violaine Moreau

PROVIDER: PXD043640 | Pride | 2024-01-26

REPOSITORIES: Pride

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Loss of RND3/RHOE controls entosis through LAMP1 expression in hepatocellular carcinoma.

Basbous Sara S   Dif Lydia L   Dantzer Camille C   Di-Tommaso Sylvaine S   Dupuy Jean-William JW   Bioulac-Sage Paulette P   Raymond Anne-Aurélie AA   Desdouets Chantal C   Saltel Frédéric F   Moreau Violaine V  

Cell death & disease 20240113 1

Entosis is a process that leads to the formation of cell-in-cell structures commonly found in cancers. Here, we identified entosis in hepatocellular carcinoma and the loss of Rnd3 (also known as RhoE) as an efficient inducer of this mechanism. We characterized the different stages and the molecular regulators of entosis induced after Rnd3 silencing. We demonstrated that this process depends on the RhoA/ROCK pathway, but not on E-cadherin. The proteomic profiling of entotic cells allowed us to id  ...[more]

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