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Whole proteome of MDK model mice

ABSTRACT: MDK is a heparin-binding growth factor and found to be the Aβ associated protein. AD mouse model of MDK knockout was established to provide the animal tool to investigate the effect of MDK on AD. Through proteome, we aim to study the Aβ change and identify the DE proteins by MDK knockout to know how MDK affect AD.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Shu Yang

LAB HEAD: Junmin Peng

PROVIDER: PXD046539 | Pride | 2025-07-16

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	checksum.txt	Txt
	mTMT70_F1.1.pepXML	Pepxml
	mTMT70_F1.raw	Raw
	mTMT70_F10.1.pepXML	Pepxml
	mTMT70_F10.raw	Raw

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Publications

Midkine Attenuates Aβ Fibril Assembly and Amyloid Plaque Formation.

Zaman Masihuz M Yang Shu S Huang Ya Y Yarbro Jay M JM Hao Yanhong Y Wang Zhen Z Liu Danting D Harper Kiara E KE Soliman Hadeer H Hemphill Alex A Harvey Sarah S Pruett-Miller Shondra M SM Stewart Valerie V Tanwar Ajay Singh AS Kalathur Ravi R Grace Christy R CR Turk Martin M Chittori Sagar S Jiao Yun Y Wu Zhiping Z High Anthony A AA Wang Xusheng X Serrano Geidy E GE Beach Thomas G TG Yu Gang G Yang Yang Y Chen Ping-Chung PC Peng Junmin J

bioRxiv : the preprint server for biology 20250320

Proteomic profiling of Alzheimer's disease (AD) brains has identified numerous understudied proteins, including midkine (MDK), that are highly upregulated and correlated with Aβ since the early disease stage, but their roles in disease progression are not fully understood. Here we present that MDK attenuates Aβ assembly and influences amyloid formation in the 5xFAD amyloidosis mouse model. MDK protein mitigates fibril formation of both Aβ40 and Aβ42 peptides in Thioflavin T fluorescence assay, c ...[more]

PMID: 40166321

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Aggregated proteome of MDK model mice

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Global Profiling of Lysine Accessibility to Evaluate Protein Structure Changes in Alzheimer’s Disease

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Ultra Deep Mouse cerebrospinal fluid (CSF) profiling in Alzheimer’s disease

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2019-04-23 | PXD011482 | Pride

Deep serum proteomics detects sex-influenced signature in a murine model of Alzheimer's disease

Project description:Alzheimer’s disease (AD) is the most common neurodegenerative disorder and is categorized by the deposition of extracellular amyloid plaques and intracellular neurofibrillary tangles. By using mouse models and performing In-depth analysis of the serum proteome has the potential to identify biomarkers that would allow for both early disease detection and measurement of disease progression. Here, we present an ultra-deep, multiplexed, undepleted mouse serum proteome by combining TMT pro-16-plex tandem-mass-tag (TMT)-based labeling, exhaustive two-dimensional liquid chromatography (LC/LC) fractionation, and sample concatenation based on the peptide concentration intensity (low, medium, and high) coupled with high resolution tandem mass spectrometry (MS/MS). We optimized a platform capable of analyzing 4100 protein components (3788 genes), covering a dynamic range of at least 6 orders of magnitude, representing one of the deepest serum proteome analyses by extensive fractionation and concatenation to date. Differential expression (DE) analysis between WT and 5xFAD mouse models showed essentially no protein changes except Lcn11. Interesting, our method detected 65 sex-dependent DE proteins with statistical significance (FDR < 0.05), including some earlier reported molecules related towards AD disease progression Serpina3h, Serpina3c, A1bg, Ncam2, Fgl1, Hamp, Kif15, Man2b2, Egfr, Prl etc, had adequate sensitivity and reproducibility to detect sex-dependent differentially expressed proteins in disease-related pathways. Hence, our newly developed protocol revealed alterations in key sex-related proteins that can be differentiated in serum analysis, both known and novel, which may be relevant to the development of assays for the monitoring and/or treatment various diseases.

2024-05-21 | PXD044306 | Pride

Profiling of the detergent-insoluble proteome of the cortical brain tissues by TMT-LC/LC-MS/MS

Project description:Mass spectrometry analysis of insoluble proteome from 5xFAD/Mdk-KO mouse brain. This study examines the insoluble protein fraction, focusing on aggregation-prone proteins including Aβ and its associated proteins. The data reveals significant accumulation of Aβ-correlated proteins and microglial components in the absence of MDK, supporting its protective role against amyloid pathology in Alzheimer's disease.

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