Dataset Information

Global proteome characterization of a three-dimensional human chondrocyte cell culture system during chikungunya virus (CHIKV) infection reveals distinct immune and inflammatory signatures

ABSTRACT: Chikungunya virus (CHIKV) belongs to alphavirus infections which often tend to deliberate musculoskeletal disorders affecting joints, muscles, and bones. Primary cells isolated from patients undergoing total knee replacement were utilized to perceive the effect of virus infection on it, upon isolation and characterization. A well-characterized set of cells was utilized to set the model system in a 2D monolayer and a 3D spheroidal model which was used to evaluate the infection profile of primary chondrocyte cells to CHIKV, the model systems were infected at different multiplicity of infection (MOI) of 0.1, 1 over number of days. Viral titers were determined by plaque assay. We further obtained the global proteome of the infected chondrocytes in its 2D and 3D architecture and analysed various pathways and perturbed proteins in two model systems. Gene expression studies were conducted using total RNA of isolated from cells to study the articular genes upon infection. Our study demonstrates that human chondrocyte cells are permissive to CHIKV infection which is evaluated in both 2D model system and 3D model systems, a comparative study of the proteomes of the chondrocytes was obtained from both 2D and 3D cultures in which distinct protein signatures attributing to cell architecture and immune system were detected.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Sujatha Sunil

LAB HEAD: Dr. Sujatha Sunil

PROVIDER: PXD055872 | Pride | 2025-10-06

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2D_ABDUL_HASAN_ICGEB_ANALYSIS_201021.pdResult	Other
	3D_ABDUL_HASAN_ICGEB_ANALYSIS_13052022.pdResult	Other
	HC-I1.raw	Raw
	HC-I2.raw	Raw
	HC-I3.raw	Raw

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Publications

Global Proteome Analysis of a Three-Dimensional Human Chondrocyte Cell Culture System Infected with Chikungunya Virus (CHIKV) Reveals Distinct Immune and Inflammatory Signatures.

Hasan Abdul A Sharma Geetika G Majumder Nilotpal N Chaudhary Sakshi S Mehta Divya D Vaishya Raju R Ghosh Sourabh S Sunil Sujatha S

ACS omega 20250910 37

Chikungunya disease (CHIKD) presents as an acute viral fever with severe joint pain, which may progress into a chronic arthritic condition that can last for months or even years. The cell tropism of chikungunya virus (CHIKV) infection is well studied; however, the mechanisms underlying the pathogenesis of chronicity, especially in the joints, remain poorly understood. Primary chondrocytes were isolated from human joint samples, characterized and grown both as monolayers and as 3D spheroids. Thes ...[more]

PMID: 41018649

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