Dataset Information

NRBP1 pseudokinase binds to and activates the WNK pathway in response to osmotic stress - Figure 1D/S1C

ABSTRACT: Please refer to the manuscript for a detailed description.

INSTRUMENT(S): Orbitrap Astral

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Frederic Lamoliatte

LAB HEAD: Dario R. Alessi

PROVIDER: PXD062616 | Pride | 2025-07-21

REPOSITORIES: Pride

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			Action	DRS
	20241023_FL_Ram_DA_DiaNN.zip	Other
	20241023_FL_Ram_DA_Gm_1.raw	Raw
	20241023_FL_Ram_DA_Gm_2.raw	Raw
	20241023_FL_Ram_DA_Gm_3.raw	Raw
	20241023_FL_Ram_DA_Gm_4.raw	Raw

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NRBP1 pseudokinase binds to and activates the WNK pathway in response to osmotic stress.

Amnekar Ramchandra V RV Dite Toby T Lis Pawel P Bell Sebastian S Brown Fiona F Johnson Clare C Wilkinson Stuart S Raggett Samantha S Dorward Mark M Wightman Mel M Macartney Thomas T Soares Renata F RF Lamoliatte Frederic F Alessi Dario R DR

Science advances 20250716 29

WNK family kinases are regulated by osmotic stress and control ion homeostasis by activating SPAK and OXSR1 kinases. Using a proximity labeling approach, we found that osmotic stress promotes the association of WNK1 with the NRBP1 pseudokinase and TSC22D2/4 adaptor proteins, results that are confirmed by immunoprecipitation, mass spectrometry, and immunoblotting studies. NRBP1 pseudokinase is closely related to WNK isoforms and contains a RΦ-motif-binding conserved C-terminal (CCT) domain, like ...[more]

PMID: 40668933

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