Dataset Information

Label-free proteome analysis of pancreatic cancer cells exposed to 2DG

ABSTRACT: The glycolytic inhibitor 2-deoxy-d-glucose (2DG) causes energy starvation, affecting cell viability in a wide range of cancer cell lines. To determine the action of 2DG in pancreatic cancer, we performed proteomic analysis of pancreatic cancer cell line after 2DG treatment. Up-regulation of glutamine: fructose 6-phosphate aminotransferase 1 (GFAT1), which belongs to the hexosamine biosynthesis pathway (HBP) that produces uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) to maintain glycoprotein, was validated by evaluation of mRNA and protein levels.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Pancreatic Ductal Adenocarcinoma

SUBMITTER: Kousuke Ishino

LAB HEAD: Naito Zenya

PROVIDER: PXD070833 | Pride | 2025-11-17

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	001_140620_Dose-dependent24h_10mM2DG_140331M24hpos_5uL500ng_3.mgf	Mgf
	001_140620_Dose-dependent24h_10mM2DG_140331M24hpos_5uL500ng_3.wiff	Wiff
	001_140620_Dose-dependent24h_10mM2DG_140331M24hpos_5uL500ng_3.wiff.scan	Wiff
	005_140620_Dose-dependent24h_0mM2DG_140331M24h-_5uL500ng_3.mgf	Mgf
	005_140620_Dose-dependent24h_0mM2DG_140331M24h-_5uL500ng_3.wiff	Wiff

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Publications

2-Deoxy-d-glucose increases GFAT1 phosphorylation resulting in endoplasmic reticulum-related apoptosis via disruption of protein N-glycosylation in pancreatic cancer cells.

Ishino Kousuke K Kudo Mitsuhiro M Peng Wei-Xia WX Kure Shoko S Kawahara Kiyoko K Teduka Kiyoshi K Kawamoto Yoko Y Kitamura Taeko T Fujii Takenori T Yamamoto Tetsushi T Wada Ryuichi R Naito Zenya Z

Biochemical and biophysical research communications 20180601 3

The glycolytic inhibitor 2-deoxy-d-glucose (2DG) causes energy starvation, affecting cell viability in a wide range of cancer cell lines. To determine the action of 2DG in pancreatic cancer, we performed proteomic analysis of pancreatic cancer cell line after 2DG treatment. Eighty proteins showed differential expression and among these, proteins involved in phosphohexose metabolism were upregulated. Up-regulation of glutamine: fructose 6-phosphate aminotransferase 1 (GFAT1), which belongs to the ...[more]

PMID: 29753740

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