Proteomics

Dataset Information

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Proteomic screen of PACS1 interactors in HCT116 cells

ABSTRACT: PACS1 syndrome is a neurodevelopmental disorder caused by a recurrent heterozygous missense mutation in PACS1 (p.R203W). We previously showed that PACS1R203W aberrantly potentiates HDAC6 activity, leading to Golgi fragmentation and neuronal deficits through an unresolved mechanism. To elucidate how PACS1R203W and HDAC6 disrupt Golgi positioning, we performed a proteomic screen to identify additional PACS1R203W partners involved in microtubule-based trafficking. HCT116 cells expressing FLAG-tagged PACS1R203W or PACS1 (control) underwent anti-FLAG immunoprecipitation (IP), and the co-purified proteins were analyzed by tandem mass spectrometry (LC-MS/MS).

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Gary Thomas  

LAB HEAD: Gary Thomas

PROVIDER: PXD074970 | Pride | 2026-04-06

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
TQ1_080219_03_pcDNA_section_1_all_entries.group Other
TQ1_080219_03_pcDNA_section_1_all_entries.mgf Mgf
TQ1_080219_03_pcDNA_section_1_all_entries.wiff Wiff
TQ1_080219_03_pcDNA_section_1_all_entries.wiff.scan Wiff
TQ1_080219_06_PACS1_WT_section_2_all_entries.group Other
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Publications

PACS1 syndrome is a neurodevelopmental disorder caused by a recurrent heterozygous missense mutation in PACS1 (p.R203W). We previously showed that PACS1<sup>R203W</sup> aberrantly potentiates HDAC6 activity, leading to Golgi fragmentation and neuronal deficits through an unresolved mechanism (Villar-Pazos and Thomas et al., Nature Commun. 14:6547-6564 (2023)). Here, we identify cytoplasmic dynein-1 heavy chain (DHC1) as a PACS1 interactor essential for maintaining furin localization at the trans  ...[more]

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