Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MS-based proteomic analysis of TgGCN5b-dependent acetylome and proteome

ABSTRACT: Histone acetylation, a fundamental epigenetic mechanism that controls gene activity, is essential for the developmental plasticity and virulence of the parasite Toxoplasma gondii. However, how this parasite organizes and deploys its acetyltransferase machinery has remained unclear. We show that T. gondii rewired this process using a plant-like system built around the acetyltransferase TgGCN5b, which differs from the typical SAGA complex found in other eukaryotes. We evaluated the impact of TgGCN5b depletion on lysine acetylome and total proteome of infecting parasites.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Toxoplasma Gondii

SUBMITTER: Yohann Couté

LAB HEAD: Yohann Couté

PROVIDER: PXD079432 | Pride | 2026-07-25

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	AcK_IAA-R1.mgf	Mgf
	AcK_IAA-R1.raw	Raw
	AcK_IAA-R2.mgf	Mgf
	AcK_IAA-R2.raw	Raw
	AcK_IAA-R3.mgf	Mgf

Items per page:

1 - 5 of 21

Similar Datasets

Mass spectrometry-based proteomic characterization of TgGCN5b and TgGNAT2 interactomes

Project description:Histone acetylation, a fundamental epigenetic mechanism that controls gene activity, is essential for the developmental plasticity and virulence of the parasite Toxoplasma gondii. However, how this parasite organizes and deploys its acetyltransferase machinery has remained unclear. We show that T. gondii rewired this process using a plant-like system built around the acetyltransferase TgGCN5b, which differs from the typical SAGA complex found in other eukaryotes. We complete our interactome analysis by characterizing TgGCN5b interactome in PHD1 KD or AP2XI-2 and AP2XII-1 KD parasites, TgGCN5b interactome from simple and double purifications, and TgGNAT2 interactome.

2026-07-25 | PXD079431 | Pride

Mass spectrometry-based proteomic characterization of TgGCN5b, TgPHD1, TgPZP1, TgGNAT1 and TgGNAT2 interactomes

Project description:Histone acetylation, a fundamental epigenetic mechanism that controls gene activity, is essential for the developmental plasticity and virulence of the parasite Toxoplasma gondii. However, how this parasite organizes and deploys its acetyltransferase machinery has remained unclear. Here, we show that T. gondii rewired this process using a plant-like system built around the acetyltransferase TgGCN5b, which differs from the typical SAGA complex found in other eukaryotes.

2026-07-25 | PXD072342 | Pride

In vitro production of cat-restricted Toxoplasma pre-sexual stages by epigenetic reprogramming

Project description:Sexual reproduction of Toxoplasma gondii, which is restricted to the small intestine of felids, is sparsely documented in studies involving domestic cats, which also raises ethical concerns. Chromatin modifiers dictate the developmental fate of the parasite during its multistage life cycle, but their targeting to stage-specific cistromes is poorly described. Here we show that AP2XII-1 and AP2XI-2, two transcription factors expressed in tachyzoites, a stage that causes acute toxoplasmosis, silence genes specific to merozoites, a developmental stage critical for sexual commitment and transmission to the next host, including humans. Their conditional and simultaneous depletion leads to a drastic change in the transcriptional program, promoting a complete transition from tachyzoites to merozoites, as revealed by mass spectrometry-based proteomic analyses of the parasites.

2023-09-16 | PXD039400 | Pride

Label-free Quantitative Acetylomics Revealed Toxoplasma gondii Genotype-Specific Acetylome Signatures

Project description:Distinct genotypic and pathogenicity differences exist between strains of the opportunistic protozoan Toxoplasma gondii. The label-free quantitative acetylomics approach was applied to investigate the differences in the level of acetylation between RH strain, PRU strain and PYS strain of T. gondii. The results showed that lysine acetylation in RH strain was the largest (458 acetylated proteins) among the three strains. The PYS strain had the second-largest acetylome (188 acetylated proteins), whereas lysine acetylation in PRU strain (115 acetylated proteins) was the smallest. Motif analysis revealed four, three and four motifs enriched from lysine acetylation sequences in RH strain, PRU strain and PYS strain respectively, suggesting specificity of acetyltransferases in these strains. Our analysis also identified 15 DAPs (differentially expressed acetylated proteins), 3 DAPs and 26 DAPs in RH strain vs PRU strain, PRU strain vs PYS strain and PYS strain vs RH strain, respectively. Compared with PYS strain and PRU strain, histone acetyltransferase and glycyl-tRNA synthase had higher expression levels in RH strain, reflecting genotype-specific differences in stress tolerance. These findings provide novel insight into the acetylomic profiles of major genotypes of T. gondii and provide an important resource for further analysis of the roles of the acetylated parasite proteins in the regulation of cellular functions.

2020-05-27 | PXD015031 | Pride

The transcription factor AP2XI-2 is a key negative regulator of Toxoplasma gondii merogony

Project description:Commitment to and completion of sexual development are essential for Toxoplasma gondii to produce oocysts in the intestine of the feline host. Understanding of the molecular mechanism responsible for sexual commitment is extremely limited due to the lack of model systems. Here, we show that the transcription factors AP2XI-2 and AP2XII-1 associated with the epigenetic repressors MORC/HDAC3 complex are constitutively expressed in both tachyzoite and bradyzoite stages but not in the merozoite stage. Depletion of AP2XI-2 or AP2XII-1 elicits the expression of genes specific to merozoites, but they play different roles in the merogony process. Depletion of AP2XI-2 in type II Pru strain induced parasites to undergo merogony and produce mature merozoites in alkaline medium but not in neutral medium, whereas the AP2XII-1 depleted Pru strain underwent several rounds of schizogony and produced merozoites in neutral medium and more markedly under alkaline conditions. Furthermore, we show that two AP2XI-2 interacting proteins are also involved in repressing merozoite programming. Overall, these findings indicate that the merozoite primed pre-sexual commitment is controlled by an intricate regulatory network and that AP2XI-2 or AP2XII-1 depleted parasites can be used as a model to study the merogony in vitro.

2023-12-20 | PXD043808 | Pride

Elucidating the diverse roles of acetyl-CoA in the cytosol and mitochondrion of Toxoplasma gondii

Project description:Acetyl-CoA is a key metabolite in all organisms, implicated in transcriptional regulation, post-translational modification as well as fuelling the TCA cycle and the synthesis and elongation of fatty acids. The obligate intracellular parasite Toxoplasma gondii possesses two enzymes which produce acetyl-CoA in the cytosol and nucleus: the acetyl-CoA synthase (ACS) and the ATP-citrate lyase (ACL), while the branched chain α-ketoacid dehydrogenase (BCKDH) generates acetyl-CoA in the mitochondrion. Given the diverse functions of acetyl-CoA, we know little about the specific roles of distinct sub-cellular acetyl-CoA pools and how these impact overall parasite physiology. To assess the broad functions of nucleo-cytosolic as well as mitochondrial acetyl-CoA, we analysed the acetylome and total proteome of parasites lacking ACL/ACS or BCKDH.

2020-05-07 | PXD016133 | Pride

MS-based proteomic characterization of Toxoplasma gondii BCC7 interactome

Project description:Toxoplasma gondii is an obligate intracellular protozoan parasite of a wide range of homeotherm animals worldwide. T. gondii belongs to the ancient phylum of Apicomplexa and shares with the other companion groups from the Alveolata infrakingdom, a featuring trilamellar pellicle outlining the cell while a permanent apico-basal polarity also characterizes the T. gondii zoites. In addition to the plasma membrane bilayer, the pellicle also encompasses the underneath inner membrane complex (IMC) itself derived from flattened and fused vesicles identified as alveoli and made of a IMC and IMC-like protein’s repertoire, yet to complete. We identified a 500 kDa protein encoded by TGGT1_311230, the basal complex component BCC7, exhibiting a posterior localization. To gain insight about the role of BCC7 in T. gondii, we characterized its interactome.

2022-07-25 | PXD032362 | Pride

Mass spectrometry-based characterization of Toxoplasma gondii MORC interactome

Project description:T. gondii has a complex life cycle typified by an asexual development taking place in vertebrate, and a sexual reproduction which occurs exclusively in felids and thereby is less studied. The developmental transitions rely on changes in gene expression patterns, and recent studies have assigned roles for chromatin shapers, including histone modifications, in establishing specific epigenetic programs for each given stage. Here, we identified T. gondii microrchidia (MORC) protein as an upstream transcriptional repressor of sexual commitment. We performed affinity purification coupled to tandem mass spectrometry analyses to identify its binding partners.

2020-01-09 | PXD016846 | Pride

MS-based proteomic characterization of Toxoplasma gondii SNF2L, SNF2h, RFTS and RSF1d interactomes

Project description:In eukaryotic cells, ATP-dependent chromatin remodelers are specialized multiprotein machines that organize the genome and rule its accessibility by repositioning, ejecting or modifying nucleosomes, but their role in Toxoplasma gondii is still poorly understood. Phylogenetic analyzes show that the parasite retains the commonly recognized remodeler families and has evolved two divergent proteins within the ISWI family: TgSNF2h and TgSNF2L. To gain insight into the role played by these structurally divergent proteins, we characterized their interactomes and those of two associated partners, RFTS and RSF1d.

2025-02-19 | PXD056911 | Pride

Proteogenomic analysis of Toxoplasma gondii (comparing genome releases 6 and 10) to demonstrate the performance of the ProteoAnnotator software

Project description:Testing the performance of the ProteoAnnotator software via comparing analysis of annotating the Toxoplasma gondii gene models 6 and 10

2014-10-20 | PXD001042 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data