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ABSTRACT: Influence of dietary Melilotus albus on productive and physiological performance of Hu sheep

PROVIDER: PRJNA1117873 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29219108_1.fastq.gz	Fastqsanger.gz
	SRR29219108_2.fastq.gz	Fastqsanger.gz
	SRR29219109_1.fastq.gz	Fastqsanger.gz
	SRR29219109_2.fastq.gz	Fastqsanger.gz
	SRR29219110_1.fastq.gz	Fastqsanger.gz

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iTRAQ-based proteomic study investigate the effects of pre-partum dietary crude protein level on colostrum fat globule membrane proteins

Project description:Purpose: This study aimed to investigate the variation of MFGM proteins in colostrum of Hu sheep under different dietary crude protein levels. Experimental design: Collecting the colostrum from Hu sheep fed three different levels of dietary crude protein levels. Plasma membrane proteins were enriched, digested, labeled using an iTRAQ kit, and identified by LC-MS. Differentially expressed proteins were analyzed using bioinformatics technology. Results: Pair-wise comparisons among three treatments identified 286 differentially expressed MFGM proteins. Bioinformatics analysis suggested that the three most abundant differentially expressed MFGM proteins were signaling, disulfide-bonded, and secreted proteins.

2022-10-17 | PXD037465 |

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The Analysis of Genes that Influence Sheep Follicular Development by Different Nutrition Levels during the Luteal Phase using Expression Profiling

Project description:Purpose: Nutrition is an important factor that regulates reproductive performance of sheep and affects follicle development. However, the correlation between nutrition and follicle development is poorly understood at molecular level. In order to study its possible molecular mechanisms, we performed expression profiling of granulosa cells isolated from sheep that were fed with different nutrition levels during luteal phase. Methods: To do this, ewes received a maintenance diet (M) and their estrus was synchronized by intravaginal progestogen sponges for 12 days. Ewes were randomly divided into the short-term dietary restricted group (R; 0.5ÃM) and nutrient-supplemented group (S; 1.5ÃM). RNA samples were extracted from granulosa cells. Transcriptome libraries from each group were constructed by Illumina Sequencing. Results: In 18,468 detected genes, 170 genes were significantly differentially expressed; of which, 140 genes were up-regulated and 30 genes were down-regulated in group S relative to group R. These genes could be candidates regulating follicular development in sheep. GO, KEGG and Clustering analyses were performed. Conclusions: These data in our study are abundant genomic recourse to expand our understanding of the molecular and cellular events underlying follicle development. Sixteen multiparous Hu sheep (3-4 years; 42.2 Â± 1.2 kg) were randomly assigned to two groups: the short-term nutrient-supplemented group (S; n=8), and the dietary restricted group (R; n=8).Expression profiling of granulosa cells were performed by Illumina sequencing platform (HiSeq 2000).

2015-12-22 | E-GEOD-76232 | biostudies-arrayexpress

Project description:Melilotus albus whole genome

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Project description:Melilotus albus genome sequencing

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Genome-wide association study of body weights in Hu sheep

Project description:Body weight (BW) is a critical economic trait for meat production in sheep. The current study aimed to perform a genome-wide association study (GWAS) to detect significant single-nucleotide polymorphisms (SNPs) that are associated with BW in Hu sheep.

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A single-cell atlas of the single versus multiple parous Hu Sheep ovary

Project description:Follicular development is a highly coordinated process in Hu sheep. Follicle-cyclic recruitment, spatial displacement, follicle atresia, and ovulation are implicated events resulting from the somatic cells' release of molecular signals. Hu sheep is a high-quality sheep breed with high fecundity in China and is ideal for investigating high reproductive traits. In the current study, the sheep with lambing number ≥3 in three consecutive lambing records were assigned to the HLS group, and lambing number = 1 as the LLS group selected from the same farm with three consecutive lambings. Three randomly picked ewes were slaughtered within 12 h of estrus, and unilateral ovarian tissue was collected and analyzed by single-cell RNA sequencing in each group. A total of five types of somatic cells were identified, and corresponding expression profiles were mapped in the ovaries of the Hu sheep. Additionally, the results of the difference in ovary somatic cell expression profiles between HLS and LLS present that the differences between multiples vs. singleton Hu sheep were mainly clustered in the GCs. In addition, 4 granulosa cell subtypes were identified. GeneSwitches results revealed the opening of JPH1 expression and the closure of LOC101112291, which leads to different evolutionary directions of the granular cells. The expression levels of FTH1 and FTL in GCs of Hu sheep in the HLS group were significantly higher, which inhibited necroptosis and ferroptosis of mural-GCs from decreasing follicular atresia. This study constructed the cellular atlas of the ovary and revealed related biological characteristics at the cellular molecular level. It provides a theoretical basis for the mechanisms underlying the differences in ovulation numbers, which contributes to breeding high-fertility sheep and molecular genetics-based selection.

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