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Transcriptional remodeling by OTX2 directs specification and patterning of mammalian definitive endoderm [scRNA_PS_Meso]

ABSTRACT: Transcriptional remodeling by OTX2 directs specification and patterning of mammalian definitive endoderm [scRNA_PS_Meso]

PROVIDER: PRJNA1242974 | ENA |

REPOSITORIES: ENA

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Transcriptional remodeling by OTX2 directs specification and patterning of mammalian definitive endoderm.

Project description:Transcriptional remodeling by OTX2 directs specification and patterning of mammalian definitive endoderm.

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Transcriptional remodeling by OTX2 directs specification and patterning of mammalian definitive endoderm.

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Transcriptional remodeling by OTX2 directs specification and patterning of mammalian definitive endoderm [CUTnTAG]

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Transcriptional remodeling by OTX2 directs specification and patterning of mammalian definitive endoderm [CUT&RUN]

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Transcriptional remodeling by OTX2 directs specification and patterning of mammalian definitive endoderm [CUTnTAG]

Project description:We discovered a role for the transcription factor OTX2 in formation of mouse definitive endoderm (DE). We used CUT&TAG to map H3K27ac- and H3K4me1-bound genomic regions and identify OTX2-bound enhancers during directed differentiation of EpiSCs to Primitive Streak (PS) intermediates and DE

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Transcriptional remodeling by OTX2 directs specification and patterning of mammalian definitive endoderm [scRNA_PS_Meso]

Project description:We discovered a role for the transcription factor OTX2 in formation of mouse definitive endoderm (DE). We determined the gene expression profiles during conversion of EpiSCs to Primitive Streak (PS) and Paraxial Mesoderm (PM) using scRNA-seq. We used dTAG13 to deplete OTX2 at PS.

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Transcriptional remodeling by OTX2 directs specification and patterning of mammalian definitive endoderm [scRNA-seq]

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