Project description:In isolated glomeruli by beads-perfusion methods, microRNA (miRNA) expression was analyzed in healthy C57BL/6 and B6.MRLc1 glomerulonephritis mice. The expression of 1,135 miRNAs was examined, and up-regulated or down-regulated miRNA was determined. These results provide a basical information of molecular pathology in glomerulonephritis.
Project description:In isolated glomeruli by beads-perfusion methods, microRNA (miRNA) expression was analyzed in healthy C57BL/6 and B6.MRLc1 glomerulonephritis mice. The expression of 1,135 miRNAs was examined, and up-regulated or down-regulated miRNA was determined. These results provide a basical information of molecular pathology in glomerulonephritis. The glomeruli were collected from female C57BL/6 (n=3, 9-month-old, healthy control) and female B6.MRLc1 (n=3, 14-month-old, early stage of glomerulonephritis; n=3, 9-month-old, late stage of glomerulonephritis). The total RNA sample was purified, and total 1,135 microRNA expression was compared among healthy, early stage, and late stage.
Project description:We collected whole genome testis expression data from hybrid zone mice. We integrated GWAS mapping of testis expression traits and low testis weight to gain insight into the genetic basis of hybrid male sterility.
Project description:To investigate the differences in microRNA expression profiles between fibrotic and normal livers, we performed microRNA microarrays for total RNA extracts isolated from mouse livers treated with carbontetrachloride (CCl4) or corn-oil for 10 weeks (n=3/group). MicroRNAs were considered to have significant differences in expression level when the expression difference showed more than two-fold change between the experimental and control groups at p<0.05. We found that 12 miRNAs were differentially expressed in CCl4-induced fibrotic liver.
Project description:CD69 is a transmembrane protein expressed on the surface of activated leukocyte. The ligand for CD69 and the intracellular signaling pathway of this molecule are yet unknown. It is widely used as a marker of activated lymphocyte, but its function in immune system is not known. We used micro-array to define genes whose expression is regulated by activation antigene CD69. CD4 T cells were isolated from the spleen of wt B6 and CD69-deficient B6 mice and in vitro activated with anti-CD3/anti-CD28 coated beads. On one groupe of wt B6 cells, CD69 was activated using a anti-CD69 and secoundary antibody. RNA extraction and hybridization on Affymetrix microarrays was performed for wt B6, CD69-activated wt B6 and CD69-deficient B6 CD4 T cells.
