Project description:Total RNA sequencing was performed on samples isolated from joint synovial biopsies from subjects with and without rheumatoid arthritis
Project description:Total RNA sequencing was performed on samples isolated from joint synovial biopsies from subjects with rheumatoid arthritis before and after 6 months of triple DMARD treatment
Project description:Gene expression profiles in synovial biopsies from patients with rheumatoid arthritis (RA) display a high level of plasticity related to disease activity and response to therapy. In order to identify TNFa-dependent genes in ex vivo RA or other synovial biopsies, we generated the present set of data using primary fibroblast-like synovial cell (FLS) cultures incubated overnight in the presence or the absence of TNFa.
Project description:This dataset contains processed 10x Genomics single-cell RNA-seq data from synovial fluid cells of three treatment-naive osteoarthritis (OA) patients. These OA samples were used as controls in the published Nature Communications study "Single cell immunoprofile of synovial fluid in rheumatoid arthritis with TNF/JAK inhibitor treatment" (doi: 10.1038/s41467-025-57361-0), which profiled synovial fluid immune cells to characterize rheumatoid arthritis-associated cellular states and treatment-related immune remodeling.
Project description:Synovial biopsies of Rheumatoid Arthritis patients with active disease were obtained prior to anti-TNF therapy. Clinical response to anti-TNF treatment was measured 20 weeks later using the EULAR response criteria. Gene expression profiles of patients responding to anti-TNF therapy were compared to non-responders and several genes were found to be differentially expressed between both groups of Rheumatoid Arthritis patients.
