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Mus musculus

ABSTRACT: anti-NONO RIP seq in liver nuclei after fasting and re-feeding

PROVIDER: PRJNA383792 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5464190.fastq.gz	Fastqsanger.gz
	SRR5464183.fastq.gz	Fastqsanger.gz
	SRR5464184.fastq.gz	Fastqsanger.gz
	SRR5464185.fastq.gz	Fastqsanger.gz
	SRR5464186.fastq.gz	Fastqsanger.gz

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anti-NONO RIP seq in liver nuclei after fasting and re-feeding

Project description:In order to identify the RNAs bound by NONO complexes in different nutritional conditions, we carried out RNA immunoprecipitation followed by sequencing (RIP-seq) of NONO from mouse liver nuclei collected at three different times of the day. Samples were collected after fasting (ZT10), 2h after re-feeding (ZT14) and towards the end of the feeding period (ZT22). The co-immunoprecipitated RNA (along with input RNA and unspecific IgG bound RNA) was sequenced.

2018-01-18 | GSE98040 | GEO

Diurnal liver transcriptome in wt and nono-/- mice

Project description:The liver circadian transcriptome results from the combined action of a circadian clock and feeding/fasting rhythms. Since we found that (1) the number of NONO-containing speckles increases in response to feeding, (2) NONO interacts with RNA processing factors and (3) it binds to the introns of a large number of protein coding genes, we hypothesized that NONO may contribute to the daily rhythm of hepatic gene expression. To address whether NONO contributes to rhythmic gene expression in response to feeding/fasting, diurnal liver transcriptomes were assessed in nono-/- and wt littermate mice fed a normal diet during the night time (ZT12-24). We habituated mice to a 12:12 LD cycle with food available only at lights off (ZT12-24) for one week and in the second week collected mice every 2h throughout the 24h day. Both total and nuclear RNAs were sequenced after ribosomal RNA depletion

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Mouse Hepatic Lipid Droplet Proteome under Fasting and Re-Feeding

Project description:One of the key functions of the mammalian liver is lipid metabolism. During fasting, lipid storage in the liver increases in order to reserve and provide energy for cellular functions. Upon re-feeding, this reserve of lipids is rapidly depleted; this change is visible, as the organelles responsible for lipid storage – lipid droplets (LDs) – drastically decrease in size following re-feeding. Little is known regarding LD proteome, or how it changes during the fasting/re-feeding transition. Our study investigated the hepatic LD proteome and how it changes between fasting and re-feeding conditions. For this purpose, LDs were isolated from 4 month-old C57BL/6 mice after a 24 hour fasting period, or a 24 hour fasting period followed by 6 hours of re-feeding. Proteins isolated from these LDs were subject to SDS-PAGE followed by in-gel trypsinization and LC-MS/MS. We identified a combined total of 941 proteins on hepatic LDs, of which 817 had quantifiable extracted ion chromatograms in at least 2 samples (n=6 total) and were not deemed contaminants. 777 of the 817 proteins were observed in both energetic states, with 33 being uniquely observed in fasted LDs, and 7 being uniquely observed in re-fed LDs.

2018-05-25 | PXD005977 | Pride

ATAC-sequencing to evaluate the global chromatin dynamics in the absence of hepatic-HMGB1 in livers from mice on fed with chow diet or after a metabolic stress induced by a fasting (6hours)-refeeding (8hours) challenge.

Project description:Hepatocyte nuclei were purified from liver samples harvested from HMGB1fl/fl (n=2) and HMGB1ΔHep (n=2) mice upon chow diet feeding or after fasting-refeeding.

2022-03-31 | E-MTAB-11542 | biostudies-arrayexpress

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2023-03-07 | GSE217597 | GEO

Characterizing phenotypic data of Peromyscus leucopus compared to C57BL/6J Mus musculus and diversity outbred (DO) Mus musculus.

Project description:Translational research is commonly performed in the C57B6/J mouse strain, chosen for its genetic homogeneity and phenotypic uniformity. Here, we evaluate the suitability of the white-footed deer mouse (Peromyscus leucopus) as a model organism for aging research, offering a comparative analysis against C57B6/J and diversity outbred (DO) Mus musculus strains. Our study includes comparisons of body composition, skeletal muscle function, and cardiovascular parameters, shedding light on potential applications and limitations of P. leucopus in aging studies. Notably, P. leucopus exhibits distinct body composition characteristics, emphasizing reduced muscle force exertion and a unique metabolism, particularly in fat mass. Cardiovascular assessments showed changes in arterial stiffness, challenging conventional assumptions and highlighting the need for a nuanced interpretation of aging-related phenotypes. Our study also highlights inherent challenges associated with maintaining and phenotyping P. leucopus cohorts. Behavioral considerations, including anxiety-induced responses during handling and phenotyping assessment, pose obstacles in acquiring meaningful data. Moreover, the unique anatomy of P. leucopus necessitates careful adaptation of protocols designed for Mus musculus. While showcasing potential benefits, further extensive analyses across broader age ranges and larger cohorts are necessary to establish the reliability of P. leucopus as a robust and translatable model for aging studies.

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