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Transcriptomic profiling of human corona virus (HCoV)-229E -infected human cells

ABSTRACT: Transcriptomic profiling of human corona virus (HCoV)-229E -infected human cells

PROVIDER: PRJNA656274 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12424243.fastq.gz	Fastqsanger.gz
	SRR12424244.fastq.gz	Fastqsanger.gz
	SRR12424245.fastq.gz	Fastqsanger.gz
	SRR12424246.fastq.gz	Fastqsanger.gz
	SRR12424247.fastq.gz	Fastqsanger.gz

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Transcriptomic profiling of human corona virus (HCoV)-229E -infected human cells

Project description:Human coronaviruses (HCoVs) cause mild to severe respiratory infection. Most of the common cold illnesses are caused by one of four HCoVs, namely HCoV-229E, HCoV-NL63, HCoV-HKU1 and HCoV-OC43. Several studies have applied global transcriptomic methods to understand host responses to HCoV infection, with most studies focusing on the pandemic severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome CoV (MERS-CoV) and the newly emerging SARS-CoV-2. In this study, Next Generation Sequencing was used to gain new insights into cellular transcriptomic changes elicited by alphacoronavirus HCoV-229E. HCoV-229E-infected MRC5 cells showed marked downregulation of superpathway of cholesterol biosynthesis and eIF2 signaling pathways. Moreover, upregulation of cyclins, cell cycle control of chromosomal replication, and role of BRCA1 in DNA damage response, alongside downregulation of the cell cycle G1/S checkpoint, suggest that HCoV-229E favors S phase for viral infection. Intriguingly, more than 80% of key factors of cell innate immunity, interferon-stimulated genes (ISGs) and other transcripts of early antiviral response genes were downregulated early in HCoV-229E infection. This study will enhance our understanding of commonly circulating HCoVs and hopefully provide critical information about still-emerging coronaviruses.

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HCoV-229E infection of the human HuH7 cell line [Experiment A]

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Time-course of HCoV-229E infection of A549 cells

Project description:The aim of the experiment is to identify kinetic changes in host gene expression upon infection of A549 lung epithelial carcinoma cells with human corona virus HCoV-229E as compared to unifected cells. Negativ controls include infections with heat-inactivated virus. Positive controls include treatment of cells with human IL-1alpha (10ng/ml) for 1h. All experiments have been performed at 33°C which is required for sufficient viral entry/replication.

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Project description:The purpose of this experiment was to evaluate the human host cellular response to wild-type Human coronavirus strain 229E (HCoV-229E) infection. Sample data was obtained for mock and infected (MOI 5) immortalized human lung epithelial cells (A549) and immortalized human lung fibroblasts cells (MRC5) processed for RNA sequencing (RNA-Seq) expression analysis.

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Human Primary Airway Epithelium Response to HCoV-229E Infection in the Presence and Absence of Human Macrophages

Project description:The purpose of this experiment was to evaluate the human host cellular response to wild-type Human coronavirus strain 229E (HCoV-229E) infection in the presence and absence of macrophages. Sample data was obtained for mock and infected (MOI 3) primary human airway epithelial cells with and without macrophages and grown in air-liquid interface conditions, processed for RNA sequencing (RNA-Seq) expression analysis.

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Project description:In this study, we define ZBTB7A as a driver of cellular survival during HCoV-229E infection.

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ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection II

Project description:In this study, we define ZBTB7A as a driver of cellular survival during HCoV-229E infection.

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Project description:The aim of the experiment is to identify changes in host gene expression upon infection of Huh7 hepatoma carcinoma cells with human coronavirus HCoV-229E as compared to uninfected cells and in comparison to cells stimulated by IL-1alpha (10ng/ml) for 1h. All experiments have been performed at 33°C which is required for sufficient viral entry/replication. Also included are two samples which have been treated with a heat inactivated coronavirus (56°C for 5min).

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