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Proteomics20S proteasome digestion of HeLa cell extracts
The 20S proteasome is responsible for the catalytic activity of all proteasome complexes. Structural constraints mean that only unfolded, extended polypeptide chains may enter the catalytic core of the 20S proteasome. Here we conducted a comprehensive analysis of the 20S CP substrates in-vitro. We r...
ORGANISM(S): Homo sapiens (Human) 
2018-07-30 | PXD010132 | Pride
20s proteasome digestion Heat soluble proteins 
ProteomicsUbiquitin remenants identification following in vitro assay of 20S and 26S proteasome using naked and ubiquitined cyclin
A systematic and comparative study of the proteolysis products generated by purified human 26S and 20S proteasome following the in vitro cleavage of non-modified (naked), mono-ubiquitinated and poly-ubiquitinated cyclin B.
ORGANISM(S): Homo sapiens (Human) 
2021-11-02 | PXD028912 | Pride
20s Ubiquitin Proteasome 
ProteomicsPTEN DEFICIENCY LEADS TO PROTEASOME ADDICTION, A NOVEL VULNERABILITY IN GLIOBLASTOMA
Glioblastoma (GBM) is the most common primary brain tumor in adults with a median survival of approximately 15 months, therefore, more effective treatment options for GBM are required. To identify new drugs targeting glioblastomas, we performed a high throughput drug screen using patient-derived neu...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-12-04 | MSV000086560 | MassIVE
ProteomicsSystematic identification of 20S proteasome targets
For many years, the ubiquitin-26S proteasome degradation pathway was considered the principal route for proteasomal degradation. However, it is now becoming clear that proteins can also be targeted for degradation by an ubiquitin-independent mechanism mediated by the core 20S proteasome itself. The ...
ORGANISM(S): Homo sapiens (Human) 
2023-12-21 | PXD044399 | Pride
Disorder Degradation 20s proteasome 
ProteomicsStructural Analysis of the 20S Proteasome using Native Mass Spectrometry and Ultraviolet Photodissociation
The yeast 20S proteasome was analyzed using native mass spectrometry combined with UVPD, HCD, and vT-ESI. Nano-LC-MS-EThcD was used to characterize the primary sequence features of the 20S subunits.
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) 
2024-11-01 | PXD056549 | JPOST Repository
ProteomicsTop-Down Analysis of 20S proteasome
Top-down LC-MS analysis of commercial and in-house immunopurified human 20S proteasome
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-07-10 | MSV000084069 | MassIVE
ProteomicsSignature cleavage in vitro assay of 20S and 26S proteasome using naked and ubiquitined cyclin
A systematic and comparative study of the proteolysis products generated by purified human 26S and 20S proteasome following the in vitro cleavage of non-modified (naked), mono-ubiquitinated and poly-ubiquitinated cyclin B.
ORGANISM(S): Homo sapiens (Human) 
2021-11-02 | PXD018711 | Pride
Human 26s proteasome 20s proteasome Cyclin 
TranscriptomicsSynergistic effects of TOR and proteasome pathways on the yeast transcriptome and cell growth
The experiments were designed to interrogate the genetic interaction relationship between the TOR signalling pathway and the UPS-proteasome pathway in gene expression and cell growth. Yeast cells (BY4742pdr5delta) were treated with the drug vehicle, MG132 (50uM), rapamycin (200ng/ml) or both drugs. ...
ORGANISM(S): Saccharomyces cerevisiae 
2013-05-24 | E-MTAB-1550 | biostudies-arrayexpress
ProteomicsSignature activities of 20S proteasome reveal degradation of intact ubiquitin-conjugates in hypoxia
Careful removal of unwanted proteins is necessary for cell survival. The primary constitutive intracellular protease is the 26S proteasome complex, very often found in equilibrium with its catalytic core particle – the 20S subcomplex. Protein degradation by the former is tightly regulated due to pri...
ORGANISM(S): Homo sapiens (Human) 
2021-11-02 | PXD018722 | Pride
26s 20s Peptides Proteasome Hypoxia 
ProteomicsKRAS G12V digestion by 20S proteasome
KRAS is a well known tumor associated antigen. It’s a GTPase that functions as molecular switch in regulatory pathways responsible for proliferation and survival. It’s frequently mutated in a variety of cancers. One of the possible driver mutations is G12V substitution, which impairs KRAS GTPase act...
ORGANISM(S): Homo sapiens (Human) 
2019-10-24 | PXD015580 | Pride
Proteasome Cancer Kras Digestion Lc-ms/ms 
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