Sort   by:  
 Page size 
ProteomicsDiscovery of highly Potent and Selective Activity-Based Probes for the Deubiquitylating Enzyme USP30
Ubiquitin-specific protease 30 (USP30) is a deubiquitylating enzyme (DUB) localized in the mitochondrial membrane, which is related to PINK1/Parkin-mediated mitophagy, pexophagy, BAX/BAK-dependent apoptosis, and IKKβ-USP30-ACLY-regulated lipogenesis/tumorigenesis. Mission therapeutics pointed their ...
ORGANISM(S): Homo sapiens (Human) 
2024-05-22 | PXD044792 | Pride
Usp30  activity-based probes  chemical probes  desi  deubiquitinase. 
ProteomicsPull down of enzymes using xylanase activity based probes (ABPs) from Bacillus subtilis secretome
This experiment aimed to identify GH5/10/30 arabinoxylan and glucuronoxylan-specific xylanases from secretomes produced by Bacillus subtilis using decorated activity based probes.
ORGANISM(S): Bacillus subtilis 
2026-06-23 | PXD065065 | Pride
Activity based probes Xylan Bacillus subtilis Xylanases 
ProteomicsPull down of enzymes using alpha-amylase activity based probes from a complex compost sample
This experiment aimed to identify new GH13 alpha-amylaases from a complex compost sample using activity based probes. The substrate specificities of these novel enzymes could also be assigned by using -1 and -2 branched activity based probes.
ORGANISM(S): Parageobacillus toebii Pseudoxanthomonas suwonensis J47 Geobacillus thermodenitrificans Aeribacillus pallidus 
2025-06-16 | PXD053848 | Pride
Alpha-amylases Tims-tof ht Activity-based probes Pull-down Glycoside hydrolase 
ProteomicsSILAC E3 ligase activity-based profiling of interferon-stimulated THP-1 cells
Activity-based probes that covalently label proteins with transthiolating E3 ligase activity were used to determine differential changes in E3 ligase activity upon interferon stimulation of monocytic THP-1 cells.
ORGANISM(S): Homo sapiens (Human) 
2026-05-22 | PXD065503 | Pride
Silac Activity-based probes 
ProteomicsPull down of enzymes using xylanase activity based probes from Clostridium thermocellum
This experiment aimed to identify GH10 xylanases from Clostridium thermocellum secretome sample using activity based probes.
ORGANISM(S): Acetivibrio thermocellus DSM 2360 
2026-06-23 | PXD065060 | Pride
Clostridium thermocellum Activity-based probes Xylanases 
ProteomicsGlycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases
Cellulases and related β-1,4-glucanases are essential components of lignocellulose-degrading enzyme mixtures. The detection of β-1,4-glucanase activity typically relies on monitoring the breakdown of purified lignocellulose-derived substrates or synthetic chromogenic substrates, limiting the activit...
ORGANISM(S): Aspergillus niger NRRL3 
2021-09-09 | PXD019930 | Pride
Abpp Activity-based probe Cellulases Cyclophellitol 
ProteomicsPull down of enzymes using alpha-amylase activity based probes from Bacteroides thetaiotaomicron lysate
This experiment aimed to identify GH13 alpha-amylaases from Bacteroides thetaiotaomicron lysate using activity based probes.
ORGANISM(S): Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482) 
2025-06-16 | PXD053850 | Pride
Alpha-amylases Tims-tof ht Activity-based probes Pull-down Glycoside hydrolase 
ProteomicsXanthohumol Activity-Based Probes Targeting Flavonoid Catabolism
Xanthohumol, the principle prenylflavonoid found in hops (Humulus lupulus), is a potential anti-inflammatory agent in the gut. To investigate the interactions between xanthohumol and catabolic gut bacteria, and its role in the anti-inflammatory pathway, a suite of xanthohumol-derived activity-based ...
ORGANISM(S): Eubacterium Ramulus (ncbitaxon:39490) Escherichia Coli (ncbitaxon:562) 
2022-02-04 | MSV000088789 | MassIVE
ProteomicsProfiling of activated RBR E3 ligases using E2V derived activity-based probes
Structure-based E2 enzyme variants (E2Vs) were used to create biotinylated activity-based probes use as handles for affinity purification-mass spectrometry to identify interacting E3s.
ORGANISM(S): Homo sapiens (Human) 
2026-01-05 | PXD068594 | Pride
E2 enzyme E3 ligase Activity-based probe 
ProteomicsABPP-HT - high-throughput activity-based profiling of deubiquitylating enzyme inhibitors in a cellular context
The potency and selectivity of a small molecule inhibitor are key parameters to assess during the early stages of drug discovery. In particular, it is very informative for characterizing compounds in a relevant cellular context in order to reveal potential off-target effects and drug efficacy. Activ...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2021-02-24 | PXD023036 | Pride
Drug discovery Pharmacology Activitomics Deubiquitylating enzymes Ubiquitin Automation Activity-based probes 
Sort   by:  
 Page size