A Flag-tagged version of murine Elongin B was expressed in mouse embryonic stem cells (ESCs) at near to endogenous levels and purified using an anti-FLAG affinity matrix.
In this project, we sought to identify the proteins interacting with the Elongin-C/Elongin-B complex. To this end, we performed affinity purification of Elongin-C and Elongin-B tagged with FLAG-STREP and MYC, respectively. A cell line with FLAG-STREP empty vector was used as negative control. 3 biol...