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MetabolomicsProteomics and tracer metabolomics link GAPDH ISGylation to glycolytic control

Ubiquitin-like protein ISG15 (interferon-stimulated gene 15) has been implicated in the regulation of central carbon metabolism, but conflicting findings across experimental systems have limited mechanistic insight. Here, we applied a multi-omics approach in cells ectopically expressing the ISGyl...

2026-03-02 | MTBLS12619 | MetaboLights
Metabolomics4-octyl itaconate inhibits aerobic glycolysis by targeting GAPDH to exert anti-inflammatory effects
Activated macrophages switch from oxidative phosphorylation to aerobic glycolysis, similar to the Warburg effect, presenting a potential therapeutic target in inflammatory disease. The endogenous metabolite itaconate has recently been reported to regulate macrophage function, but its precise mechani...
2019-10-02 | MTBLS1140 | MetaboLights
TranscriptomicsGene profile in H1299 cells treated with PTD-DRBD GAPDH siRNA or treated with Lipofection GAPDH siRNA
Whole genome microarrays were probed with total mRNA from PTD-DRBD GAPDH siRNA treated H1299 cells at 12 h and 24 h. Using a 1.6x fold increase/decrease filter of cellular mRNAs, we detected a dramatic reduction in the target GAPDH mRNA along with a limited number of both up and down regulated genes...
ORGANISM(S): Homo sapiens 
2010-05-08 | E-GEOD-15723 | biostudies-arrayexpress
ProteomicsGAPDH inhibition in A549 and H358 lung cancer cells
Human lung cancer cell lines A549 and H358 were obtained from (Biobank of Medical University of Graz) and cultured in RPMI 1640 (R0883; Sigma) supplemented with 10% fetal bovine serum (FBS; Gibco), 5 mM glutamine and 5 mM Pen Step, and sub-cultured regularly. For siRNA mediated knock-down of GAPDH, ...
ORGANISM(S): Homo sapiens (Human) 
2025-05-12 | PXD061610 | Pride
Human Koningic acid Gapdh 
ProteomicsHDX-MS data of trigger factor and GAPDH binding
We present HDX-MS data of the trigger factor:GAPDH complex to define the interaction sites for GAPDH on trigger factor and the structure of GAPDH in the bound state.
ORGANISM(S): Escherichia coli 
2022-06-07 | PXD029364 | Pride
Chaperone  trigger factor  gapdh  folding 
TranscriptomicsGlobal analysis of off-target genes by GAPDH siRNA
A GAPDH siRNA was overexpressed in HeLa cells and microarray expression profiling was performed to experimentally identify the off-targets of this siRNA at the transcriptome level. Among all predicted siRNA off-targets by MirTarget2, the majority were downregulated by at least 10% (i.e. less than 90...
ORGANISM(S): Homo sapiens 
2013-10-03 | E-GEOD-41924 | biostudies-arrayexpress
ProteomicsDIA proteomics on mouse tissue to investigate the GAPDH redox switch
The glycolytic enzyme GAPDH is equipped with a redox switch that rapidly and reversibly inactivates the enzyme in the presence of hydrogen peroxide. The physiological implications of the redox switch were investigated in C57BL/6NTac wildtype and GAPDH(T175A) knock-in mice generated by CRISPR/Cas9 ge...
ORGANISM(S): Mus musculus (Mouse) 
2023-04-10 | PXD040620 | Pride
Heart Tissue Mus musculus Kidney Gapdh Dia-pasef Mouse 
ProteomicsMapping ISG15 sites on GAPDH and PGK1 by AP-MS
We employed an AP-MS approach to map ISG15 modification sites on GAPDH and PGK1 in HEK293T cells by overexpression of FLAG-GAPDH, FLAG-PGK1 or FLAG-GFP together with the ISG15 conjugation enzymes (E1, E2, E3) and wild-type HA-ISG15 or conjugation-defective ISG15-AA. Cells were lysed in physiological...
ORGANISM(S): Homo sapiens (Human) 
2026-03-02 | PXD071724 | Pride
Ap-ms Pgk1 Gapdh Isg15 Glycolysis 
ProteomicsJ774 Macrophage proteins interacion with Paracoccidioides lutzii GAPDH
we show an overview of experimental GAPDH interactome in with macrophage proteins. Several proteins bound to GAPDH are important to stimulate host response due to infection.
ORGANISM(S): Paracoccidioides lutzii (strain ATCC MYA-826 / Pb01) (Paracoccidioides brasiliensis) 
2019-07-29 | PXD008309 | Pride
Ppi Gapdh Macrophage Paracoccidioides 
ProteomicsGAPDH interactome in micelium to yeast transition Paracoccidioides lutzii cells
we show an overview of experimental GAPDH interactome in different phases of P. lutzii. Several proteins bound to GAPDH from mycelium, transition and yeast are common to important pathways such as glycolysis and TCA.
ORGANISM(S): Paracoccidioides lutzii (strain ATCC MYA-826 / Pb01) (Paracoccidioides brasiliensis) 
2019-07-29 | PXD008317 | Pride
Gapdh Dimorphic fungi Paracoccidioides 
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