Sort   by:  
 Page size 
ProteomicsDevelopment of a high-pH reversed-phase well plate for peptide fractionation and deep proteome analysis of cells and exosomes
Development of a high-pH reversed-phase well plate for peptide fractionation and deep proteome analysis of cells and exosomes
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-07-21 | MSV000087860 | MassIVE
ProteomicsOff-line high pH reversed-phase fractionation for in-depth phosphoproteomics
In this study we provide a detailed analysis of the physicochemical characteristics of phosphopeptides, which have been fractionated by off-line high pH chromatography (HpH) before subsequent titanium dioxide (TiO2) enrichment and LC-MS/MS analysis. Our results demonstrate that HpH is superior to st...
ORGANISM(S): Mus musculus (Mouse) 
2014-10-23 | PXD001404 | Pride
Phosphoproteomics  high ph reversed-phase  phosphorylation  peptides  enrichment  fractionation  titanium dioxide  orbitrap 
ProteomicsHigh-pH Reversed-Phase Fractionated Neural Retina Proteome of Normal Growing C57BL/6 Mouse
Mouse was sacrificed at postnatal day 46 by cervical dislocation. Retina were prepared seamlessly with suspension trap (S-TRAP) followed by high-pH reversed-phase peptide fractionation. Peptides fractions were identified with data-dependent acquisition by quadrupole time-of-flight mass spectrometry.
ORGANISM(S): Mus musculus (Mouse) 
2019-09-11 | PXD014459 | Pride
Mass spectrometry Retina Mouse Proteomics 
MetabolomicsFecal microbiota transplantation plus anti-PD-1 immunotherapy in advanced melanoma: a phase I trial

Fecal microbiota transplantation (FMT) represents a potential strategy to overcome resistance to immune checkpoint inhibitors in patients with refractory melanoma; however, the role of FMT in first-line treatment settings has not been evaluated. We conducted a multicenter phase I trial combining ...

2025-02-27 | MTBLS711 | MetaboLights
ProteomicsK562 cell lysates prefractionated by various methods
K562 cell lysates were prefractionated by protein reversed-phase chromatography (Prot-RP), SDS-PAGE, and high-pH peptide RP (Pept-RP) to compare their method characteristics. The starting material was prefractionated to 10 fractions for each method. Each prefractionation method was conducted in trip...
ORGANISM(S): Homo Sapiens (human) 
2024-10-15 | PXD049312 | JPOST Repository
ProteomicsProteomic analysis of human dentin matrix extracts by peptide-level high-pH reverse-phase fractionation
Proteomics research has increasingly focused on human cells, tissues, and fluids; however, comprehensive data regarding dental tissues remain limited. Dentin, a mineralized component of the tooth, contains structural proteins and bioactive molecules that, upon release, can modulate pulp cell acti...
ORGANISM(S): Homo sapiens (Human) 
2026-03-23 | PXD070849 | Pride
Dentin  dentin matrix  proteomics  extracellular matrix  mass spectrometry  regenerative endodontics 
ProteomicsMining Missing Membrane Proteins by High-pH Reverse Phase StageTip Fractionation and Validation by Multiple Reaction Monitoring Mass Spectrometry
Several significant efforts have been made in the past decade to map all human proteome. However, more than 3000 proteins are grouped as missing having no evidence of translation. Membrane protein annotation of the current missing proteins list showed 34%to be missing membrane proteins, which are ch...
ORGANISM(S): Homo sapiens (Human) 
2015-07-27 | PXD002224 | Pride
High-ph reverse phase Stagetip Missing proteins Lung cancer 
MetabolomicsSacha inchi oil alleviates gut microbiota dysbiosis and improves hepatic lipid dysmetabolism in high-fat diet-fed rats
Dietary ω-3 polyunsaturated fatty acids (PUFAs) are beneficial for humans against the development of hyperlipidaemia, but the underlying mechanisms are still poorly understood. Here, we demonstrated that oral consumption of sacha inchi oil, which is rich in α-linolenic acid, alleviated dyslipidemia,...
2022-02-21 | MTBLS1225 | MetaboLights
ProteomicsStrong cation exchange-reversed phase liquid chromatography-capillary zone electrophoresis-tandem mass spectrometry platform with high peak capacity for deep bottom-up proteomics
Two-dimensional (2D) liquid chromatography (LC)-tandem mass spectrometry (MS/MS) are typically employed for deep bottom-up proteomics, and the state-of-the-art 2D-LC-MS/MS has approached over 8,000 protein identifications (IDs) from mammalian cell lines or tissues in 1-3 days of mass spectrometer ti...
ORGANISM(S): Mus musculus (Mouse) 
2018-02-27 | PXD008432 | Pride
Strong cation exchange  reversed-phase liquid chromatography  capillary zone electrophoresis  tandem mass spectrometry  bottom-up proteomics 
ProteomicsOnline Nanoflow Two-Dimension Comprehensive Active Modulation Reversed Phase-Reversed Phase Liquid Chromatography High Resolution Mass Spectrometry for Metaproteomics of Environmen-tal and Microbiome Samples
Metaproteomics is a powerful analytical approach that can assess the functional capabilities deployed by microbial communi-ties in both environmental and biomedical microbiome settings. Yet the mass spectra resulting from these mixed biological communities are challenging due to the high number of l...
ORGANISM(S): Homo sapiens (Human) marine plankton environmental sample 
2022-05-20 | PXD027351 | Pride
Automated Metaproteomics Proteomics Comprehensive active modulation 2dlc Rprp Lc Ocean Lcms 
Sort   by:  
 Page size