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ProteomicsAn automated intact glycopeptide enrichment method for serum site-specific N-glycoproteomic analysis
We developed an automated glycopeptide enrichment method for the analysis of serum site-specific N-glycoproteome. This automated method allowed for processing one sample within 20 min. It showed higher enrichment specificity, more intact glycopeptide identifications, and better quantitative reproduc...
ORGANISM(S): Homo Sapiens (human) 
2021-04-06 | PXD025151 | JPOST Repository
ProteomicsEffective Enrichment Strategy Using Boronic Acid Functionalized Mesoporous Graphene Silica Composites for Intact Glycopeptide Analysis in Human Serum
The heterogeneity and low abundance of protein glycosylation present challenging barriers to the analysis of intact glycopeptides, which is key to comprehensively understanding the role of glycosylation in an organism. Efficient and specific enrichment of intact glycopeptides could help greatly with...
ORGANISM(S): Human 
2021-04-28 | MSV000087324 | MassIVE
ProteomicsHuman serum N-linked intact glycopeptides LC-MS/MS
Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) in human serum is challenging due to the wide dynamic range of serum protein abundances, the lack of a complete serum N-Glycan database and the existence of non-specifical...
ORGANISM(S): Armoracia rusticana Homo sapiens (Human) Gallus gallus (Chicken) 
2020-04-21 | PXD015622 | Pride
N-linked intact glycopeptide Co-elution Spectral library search Human serum Isotopic distribution 
ProteomicsLarge scale intact glycopeptide identification by Mascot database search
We present the adaptability of Mascot search engine for automated identification of intact glycopeptide mass spectra. The steps involved in adopting Mascot for intact glycopeptide analysis include: i) assigning unique one letter codes for monosaccharides, ii) linearizing glycan sequences and iii) pr...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-07-02 | MSV000082567 | MassIVE
ProteomicspGlyco3: Intact N- and O-glycopeptide search engine
Analyses of N- and O-glycopeptide using pGlyco3 as well as Byonic, MSFragger and MetaMorpheus. N-glycopeptides and O-Man glycopeptides of fission yeast and budding yeast are also analyzed using pGlyco3.
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) Homo Sapiens (ncbitaxon:9606) Schizosaccharomyces Pombe (ncbitaxon:4896) 
2021-01-27 | MSV000086771 | MassIVE
ProteomicsAutomated N-glycopeptide identification in glycoproteomics
Recent advances in software-driven glycopeptide identification in LC-MS/MS-based N-glycoproteomics have facilitated biochemical studies reporting thousands of intact N-glycopeptides, i.e. N-glycan-conjugated peptides, but the automated identification process remains to be scrutinized. Herein, we exp...
ORGANISM(S): Homo sapiens (Human) 
2016-08-17 | PXD004243 | Pride
Basigin Byonic Glycoproteomics Glycopeptide N-glycosylation Lc-ms/ms 
ProteomicsImproving quantitative glycoproteomics by systematic evaluation of different glycopeptide enrichment, quantification, and mass spectrometry strategies
In this study, we conducted a systematic investigation into a glycoproteomic workflow. Firstly, Label-free and TMT labeling strategies were utilized for intact N-glycopeptide quantification. A total of 2,924 unique intact N-glycopeptides can be quantified after being labeled using TMT approach. Comp...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2025-05-07 | PXD054874 | Pride
Glycoproteomics Intact glycopeptide Identification Quantification 
ProteomicsLarge scale intact glycopeptide identification by Mascot database search
We present the adaptability of Mascot search engine for automated identification of intact glycopeptide mass spectra. The steps involved in adopting Mascot for intact glycopeptide analysis include: i) assigning unique one letter codes for monosaccharides, ii) linearizing glycan sequences and iii) pr...
ORGANISM(S): Homo sapiens (Human) 
2018-02-08 | PXD005931 | Pride
Mascot Glycoproteomics Higher-energy collisional induced dissociation (hcd) Prostate cancer Serum 
ProteomicsIdentification of N-Glycopeptides based on Glycocarrier Strategy
In this work, we developed a glycocarrier strategy to amplify MS signals with isobaric labeling for highly sensitive intact N-glycopeptide characterization from single and small numbers of cells without enrichment. Successful application in cell lines and microglia from the regional mouse brain demo...
ORGANISM(S): Homo Sapiens Mus Musculus 
2022-11-18 | PXD038212 |
ProteomicsIMPROVED N- AND O-GLYCOPEPTIDE IDENTIFICATION USING HIGH-FIELD ASYMMETRIC WAVEFORM ION MOBILITY SPECTROMETRY (FAIMS)
Mass spectrometry is the premier tool for identifying and quantifying site-specific protein glycosylation globally. Analysis of intact glycopeptides often requires an enrichment step, after which the samples remain highly complex and exhibit a broad dynamic range of abundance. Here, we evaluated th...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD038673 | Pride
Faims N-glycopeptides Mass spectrometry O-glycopeptides Glycoproteomics 
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