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MetabolomicsLipidomic analysis of lipid droplets from murine hepatocytes reveals distinct signatures for nutritional stress
Liver steatosis can be induced by fasting or high-fat diet. We investigated by lipidomic analysis whether such metabolic states are reflected in the lipidome of hepatocyte lipid droplets (LDs) from mice fed normal chow diet (FED), fasted (FAS), or fed a high-fat diet (HFD). LC-MS/MS at levels of lip...
2014-08-19 | MTBLS26 | MetaboLights
MetabolomicsThe impact of genetic stress by ATGL deficiency on the lipidome of lipid droplets from murine hepatocytes
We showed earlier that nutritional stress like starvation or high fat diet resulted in phenotypic changes in the lipidomes of hepatocyte lipid droplets (LDs), representative for the pathophysiological status of the mouse model. Here we extend our former study by adding genetic stress due to knock-ou...
2014-08-19 | MTBLS81 | MetaboLights
TranscriptomicsExpression data of hepatocytes isolated from chimeric mouse livers repopulated with human hepatocytes and from normal human livers
We generated chimeric mice with livers that were predominantly repopulated with human hepatocytes. Hepatocytes were isolated from the chimeric mouse livers and their gene expressions were compared with hepatocytes isolated from normal human livers . Cluster and principal components analyses showed t...
ORGANISM(S): Mus musculus 
2012-11-27 | E-GEOD-33846 | biostudies-arrayexpress
ProteomicsComparative proteomic analysis of human liver tissue and isolated hepatocytes with a focus on proteins determining drug exposure
Freshly isolated human hepatocytes are considered the gold standard for in vitro studies of liver functions, including drug transport, metabolism, and toxicity. For accurate predictions of in vivo outcome, the isolated hepatocytes should reflect the phenotype of their in vivo counterpart, i.e., hepa...
ORGANISM(S): Homo sapiens (Human) 
2015-07-16 | PXD002235 | Pride
Adme proteins Human liver Human hepatocyte Fasp 
TranscriptomicsMolecular anatomy of gene expression responses in stressed hepatocytes
It is well-known that isolation and cultivation of primary hepatocytes causes major gene expression alterations. In the present genome-wide, time resolved study of cultivated human and mouse hepatocytes, we made the observation that expression changes in culture strongly resemble alterations in live...
ORGANISM(S): Mus musculus 
2016-09-28 | E-MTAB-4444 | biostudies-arrayexpress
TranscriptomicsGlobal expression of mouse hepatocytes cultured as monolayers or as three-dimensional aggregates
Three-dimensional (3D) culture of hepatocytes leads to improved and prolonged synthetic and metabolic functions, but the underlying molecular mechanisms were unknown. In order to investigate the molecular mechanisms underlying 3D cell-cell interactions in maintaining hepatocyte differentiated funct...
ORGANISM(S): Mus musculus 
2013-12-23 | E-GEOD-53054 | biostudies-arrayexpress
TranscriptomicsBenzo[a]pyrene response in primary mouse hepatocytes
Gene expression changes to several concentrations of benzo[a]pyrene in primary hepatocytes of wildtype and Xpa-/-p53+/- mice
ORGANISM(S): Mus Musculus 
2011-08-11 | E-TABM-1139 | biostudies-arrayexpress
Transcriptomicsglobal gene expression profiles in primary human hepatocytes exposed to phytochemicals
Primary human hepatocytes were treated with 2 non-cytotoxic concentrations of sulforaphane (SFN; 10 and 50 uM) phenethyl isothiocyante (PEITC; 10 and 25 uM), 3,3'-diindolylmethane (DIM; 10 and 50 uM), or one concentration of oltipraz (OPZ; 30 uM) for 48 hours. Given the considerable heterogeneity o...
ORGANISM(S): Homo sapiens 
2010-07-19 | E-GEOD-20479 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human primary hepatocytes treated with transcription factor PPAR agonist Wy14643
Studies in mice have shown that PPARalpha is an important regulator of hepatic lipid metabolism and the acute phase response. However, little information is available on the role of PPARalpha in human liver. Here we set out to compare the function of PPARalpha in mouse and human hepatocytes via anal...
ORGANISM(S): Homo sapiens 
2009-09-25 | E-GEOD-17251 | biostudies-arrayexpress
GenomicsExpression data of hepatocytes isolated from chimeric mouse livers repopulated with human hepatocytes and from normal human livers
Expression data of hepatocytes isolated from chimeric mouse livers repopulated with human hepatocytes and from normal human livers
PRJNA148171 | ENA
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