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The recent development within high-throughput technologies for expression profiling has allowed for parallel analysis of transcriptomes and proteomes in biological systems such as comparative analysis of transcript and protein levels of tissue regulated genes. Until now, such studies of have only in...
ORGANISM(S): Sus scrofa 
The iTRAQ-LC–MS/MS strategy was used for quantitative analysis to reveal the global response of P. aeruginosa SJTD-1 to the stimuli of C18 alkane in this study. As a result, 476 proteins were identified with a significant different expression levels for the growth on n-octadecane, accounting for 8.4...
ORGANISM(S): Pseudomonas aeruginosa SJTD-1 
2022-02-22 | PXD000936 | Pride
“Dry eye” is a multi-factorial inflammatory disease affecting the ocular surface. Tear hyperosmolarity in dry eye contributes to inflammation and cell damage. Recent research efforts on dry eye have been directed towards biomarker discovery for diagnosis, response to treatment and disease mechanisms...
2015-08-31 | MTBLS214 | MetaboLights
After long-term aluminum treatment, thereafter, we investigated Citrus root protein profiles using isobaric tags for relative and absolute quantification (iTRAQ).
ORGANISM(S): Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis) Citrus maxima (Pomelo) (Citrus grandis) 
2017-10-26 | PXD002301 | Pride
AGS cells were treated with metformin then performed iTRAQ proteomics techniques.
ORGANISM(S): Homo sapiens (Human) 
2021-09-10 | PXD024996 | Pride
Stable VPS35 knockdown HeLa cells were generated using lentiviral short hairpin RNA (shRNA) construct. Isobaric tags for relative and absolute quantitation (iTRAQ)-based mass spectrometry was used to measure the changes of nuclear protein abundance in VPS35-depleted HeLa cells.
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD027166 | Pride
Secreted proteases act on interstitial tissue secretomes released from multiple cell types. Thus, substrate proteins might be part of higher molecular complexes constituted by many proteins with diverse and potentially unknown cellular origin. In cell culture these might be reconstituted by mixing n...
ORGANISM(S): Mus musculus (Mouse) 
2015-05-11 | PXD001643 | Pride
We employed UPLC-MS/MS with iTRAQ 8-plex labeling to quantitatively analyze the supernatant produced by two Chinese hamster ovary (CHO) cell lines (CHO K1SV and CHO CAT-S). In each case, the supernatant from the host and three transfected clones were analyzed at days 5, 7, and 10 of culture. A total...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2016-07-20 | PXD003462 | Pride
This study aimed to compare the depth and reproducibility of total proteome and differentially expressed protein coverage in technical duplicates and triplicates using iTRAQ 4-plex, iTRAQ 8-plex and TMT 6-plex reagents.
ORGANISM(S): Phaeosphaeria Nodorum Sn15 (ncbitaxon:321614) 
2016-03-17 | MSV000079594 | MassIVE
The effects of temperature on teleosts have been of strong interest for a long time, particularly because low temperature profoundly influences the physiological and behavioral processes of ectotherms, especially teleosts. However, the cold-tolerance characteristics of fish at a protein level remain...
ORGANISM(S): Takifugu fasciatus 
2019-05-09 | PXD010955 | Pride
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