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ProteomicsPredicting electrophoretic mobility of proteoforms for large-scale top-down proteomics
Large-scale top-down proteomics characterizes proteoforms in cells globally with high confidence and high throughput using reversed-phase liquid chromatography (RPLC)-tandem mass spectrometry (MS/MS) or capillary zone electrophoresis (CZE)-MS/MS. The false discovery rate (FDR) from the target-decoy ...
ORGANISM(S): Escherichia coli 
2020-02-17 | PXD017265 | Pride
Top-down proteomics Electrophoretic mobility Cze-ms Proteoform 
ProteomicsTop-down Proteomics of Large Proteins Up to 223 kDa Enabled by Serial Size Exclusion Chromatography Strategy
Mass spectrometry (MS)-based top-down proteomics is a powerful method for the comprehensive analysis of proteoforms that arise from genetic variations and post-translational modifications (PTMs). However, top-down MS analysis of high molecular weight (MW) proteins remains challenging mainly due to t...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-11-29 | MSV000080365 | MassIVE
UnknownIntact-Mass Analysis Facilitating the Identification of Large Human Heart Proteoforms.
Not available
S-EPMC6722015 | biostudies-literature
UnknownPredicting Electrophoretic Mobility of Proteoforms for Large-Scale Top-Down Proteomics.
Not available
S-EPMC7543059 | biostudies-literature
UnknownHigh sensitivity top-down proteomics captures single muscle cell heterogeneity in large proteoforms.
Not available
S-EPMC10175728 | biostudies-literature
UnknownProton Transfer Charge Reduction Enables High-Throughput Top-Down Analysis of Large Proteoforms.
Not available
S-EPMC7008508 | biostudies-literature
UnknownSmall-Scale Serial Size Exclusion Chromatography (s3SEC) for High Sensitivity Top-Down Proteomics of Large Proteoforms.
Not available
S-EPMC11298573 | biostudies-literature
ProteomicsSmall-scale Serial Size Exclusion Chromatography (s3SEC) for High Sensitivity Top-down Proteomics of Large Proteoforms
Mass spectrometry data for "Small-scale Serial Size Exclusion Chromatography (s3SEC) for High Sensitivity Top-down Proteomics of Large Proteoforms"
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-12-10 | MSV000093625 | MassIVE
ProteomicsCapillary zone electrophoresis-tandem mass spectrometry as an alternative to liquid chromatography-tandem mass spectrometry for top-down proteomics of histones with the identification of over 1000 histone proteoforms
Top-down proteomics (TDP) is an ideal approach for deciphering the histone code and it routinely employs reversed-phase liquid chromatography (RPLC)-tandem mass spectrometry (MS/MS). Here we present capillary zone electrophoresis (CZE)-MS/MS via the electro-kinetically pumped sheath-flow CE-MS inter...
ORGANISM(S): Bos taurus (Bovine) 
2022-04-04 | PXD021889 | Pride
Intact histone proteoforms/mobility prediction/top-down proteomics/ histone postlational modifications/calf histone 
ProteomicsMonitoring mAb proteoforms in mouse plasma using an automated immunocapture combined with top-down and middle-down mass spectrometry
We propose here a workflow based on an automated immunocapture followed by top-down and middle-down LC-MS/MS approaches to characterize mAb proteoforms spiked in mouse plasma. We demonstrate the applicability of our workflow on a large concentration range using pembrolizumab as a model. We also comp...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2024-08-27 | PXD040896 | Pride
Immunocapture Monoclonal antibody Proteoforms Middle-down mass spectrometry Top-down mass spectrometry 
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