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ProteomicsConformational control and DNA binding mechanism of the metazoan origin recognition complex
Chemical cross-linking coupled to mass spectrometry was used to study the architecture and conformational state(s) of the Drosophila melanogaster origin replication complex (ORC). Two versions of the ORC were subjected to cross-linking with the amine-reactive reagents, disuccinimidyl suberate (DSS)....
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2018-06-11 | PXD009526 | Pride
Chemical cross-linking  dss  structural proteomics  origin recognition complex Autoinhibition 
GenomicsHumanizing the Yeast Origin Recognition Complex
Humanizing the Yeast Origin Recognition Complex
PRJNA627487 | ENA
GenomicsORC precisely positions nucleosomes at origins of replication
The budding yeast genome is marked by 250-350 origins of DNA replication. These origins are bound by the origin recognition complex (ORC) throughout the cell cycle. ORC has known DNA binding sequence preferences which, though necessary for binding, are not sufficient to fully specify a genomic locus...
ORGANISM(S): Saccharomyces cerevisiae 
2010-03-19 | E-GEOD-16926 | biostudies-arrayexpress
TranscriptomicsGenome-wide localization of ORC and MCM binding sites and identification of replication origins in S. pombe
DNA replication of eukaryotic chromosomes initiates at a number of discrete loci, called replication origins. Distribution and regulation of origins are important for complete duplication of the genome. Here, we determined locations of Orc1 and Mcm6, components of pre-replicative complex (pre-RC),...
ORGANISM(S): Schizosaccharomyces pombe 
2010-06-11 | E-GEOD-6523 | biostudies-arrayexpress
GenomicsReplication licensing regulated by an intrinsically disordered region of origin recognition complex
Replication licensing regulated by an intrinsically disordered region of origin recognition complex
PRJNA1093011 | ENA
TranscriptomicsKc167 ORC2 Localization
modENCODE_submission_674 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We will precisely identify sequence elements that direct DNA replication by using chromatin immunoprecipitation of known r...
ORGANISM(S): Drosophila melanogaster 
2010-05-15 | E-GEOD-17282 | biostudies-arrayexpress
GenomicsOrdered and disordered regions of the Origin Recognition Complex direct differential in vivo binding at distinct motif sequences.
Ordered and disordered regions of the Origin Recognition Complex direct differential in vivo binding at distinct motif sequences.
PRJNA1050784 | ENA
GenomicsAnalysis of genome wide H3K9me3 levels in control and ORCA knockdown cells
Origin Recognition Complex Associated (ORCA) associates with repressive chromatin environments. We carried out H3K9me3 ChIP-seq to determine the affect of ORCA's loss on this repressive mark. Towards this end, we cultured U2OS osteosarcoma cells, performed control and ORCA knockdowns using siRNAs an...
ORGANISM(S): Homo sapiens 
2015-04-28 | E-GEOD-68129 | biostudies-arrayexpress
TranscriptomicsChIP-chip from Drosophila egg chambers using ORC2 antibody
Chromatin immunoprecipitation of the Origin Recognition Complex (ORC) followed by hybridization to genome-wide tiling microarrays demonstrated that ORC does not localize to DAFC-34B after stage 10, despite a second round of replication initiation Comparison of ORC2 ChIP sample compared to input DNA ...
ORGANISM(S): Drosophila melanogaster 
2011-09-06 | E-GEOD-31890 | biostudies-arrayexpress
GenomicsEvolution of origin sequence and recognition for licensing of Eukaryotic DNA replication
Evolution of origin sequence and recognition for licensing of Eukaryotic DNA replication
PRJNA1438573 | ENA
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