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Proteomicsp65-HaloTag copurifying proteins in human 293T cells
In effort to develop methodology for targeted top down mass spectrometry of NF kappa B p65 from human cells, we evaluated the utility of HaloTag for purification and analysis of recombinant protein. During our study, two datasets of bottom up LC-MS/MS were generated: one from in-gel digestion of the...
ORGANISM(S): Homo sapiens (Human) 
2018-10-19 | PXD001932 | Pride
Rela 293t Human Nf kappa b P65 
TranscriptomicsRibosomal protein S3: a functional component of NF-kB p65 binding complexes
To explore the effects on the NF-kB transcriptome after knockdown of RPS3 in stimulated T cells, we performed microarray assays comparing RPS3-silenced versus NS-treated samples on the same chip. p65-silenced versus NS-treated samples were used as a control. Keywords: time series design Jurkat cells...
ORGANISM(S): Homo sapiens 
2010-05-26 | E-GEOD-7231 | biostudies-arrayexpress
GenomicsGenome-wide maps of RNA Polymerase II and p65 localization in HUVECs stimulated with TNF alpha
TNF alpha is one of the inflammatory mediator and induce genes mainly by transcriptional factor, p65, in endothelial cells. This time, we performed a time course study to detect the change of localization of p65 and Pol II. To identify p65 and Pol II binding sites, we used chromatin immunoprecipit...
ORGANISM(S): Homo sapiens 
2013-01-01 | E-GEOD-34500 | biostudies-arrayexpress
ProteomicsCommensal-like vs. invasive Pneumococcus p65 interactome
Define the divergent p65 interactome between commensal-like vs. invasive pneumococcal-host interaction.
ORGANISM(S): Homo sapiens (Human) Streptococcus pneumoniae TIGR4 Streptococcus pneumoniae 670-6B 
2026-09-07 | PXD032970 | Pride
P65 Nf-kb Commd Epithelial cells Pneumococcus 
GenomicsEffects of p65 palmitoylation on gene expression when overexpressing vector or p65 WT or p65 2CS in p65 knockout 293A cells combined with TNFα treatment
Effects of p65 palmitoylation on gene expression when overexpressing vector or p65 WT or p65 2CS in p65 knockout 293A cells combined with TNFα treatment
PRJNA1209914 | ENA
GenomicsAnalysis of glucocorticoid receptor (NR3C1), NFkb (p65 subunit), and RNA polymerase 2 (RNAP2) binding in Beas-2B airway epithelial cells
The objective of this study was to determine binding patterns for GR, 65 and RNAP2 in Beas-2B airway epithelial cells after treatment with dexamethasone (100 nm), TNF (20 ng/ml) or both for one hour. This study utilized duplicate samples for each treatment condition and immunoprecipitation except fo...
ORGANISM(S): Homo sapiens 
2016-04-01 | E-GEOD-79803 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of human myometrial cells transfected by NFKB (p65) and CEBP alone and in combination.
Nuclear factor kappa B and C/EBP are two important transcription factors that independently and synergistically regulate different genes. In this experiment the expression of genes will be examined with the overexpression of NFKB (p65) and CEBP alone and in combination.
ORGANISM(S): Homo sapiens 
2010-03-24 | E-MEXP-2160 | biostudies-arrayexpress
TranscriptomicsTrap-80-dependence of TNF-alpha-induced genes
In fibroblasts, p65-dependent genes can be sub-divided, depending on whether they are Trap-80-dependent or -independent. To examine the generality of this grouping, we performed a microarray analysis of wild-type and Trap-80 knock-down fibroblasts, before and after stimulation of NF-kappaB activity...
ORGANISM(S): Mus musculus 
2010-04-09 | E-GEOD-12697 | biostudies-arrayexpress
Genomicsp65
p65
PRJNA811604 | ENA
GenomicsGenome-wide TNFM-NM-1-induced p65 binding before and after telomerase inhibition in HeLa cells
Chromatin immunoprecipitation sequencing (ChIP-seq) was performed to analyze the effect of telomerase inhibition on TNFM-NM-1-induced genome-wide p65 binding in HeLa cells. By obtaining over 40 million uniquely mappable reads per sample from ChIP-seq, maps for TNFM-NM-1-induced p65 binding in absenc...
ORGANISM(S): Homo sapiens 
2012-11-26 | E-GEOD-41100 | biostudies-arrayexpress
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