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ProteomicsEstablishment of plasmodesmata core proteome using label free semi-quantitative proteomic analysis.
To establish the plasmodesmata core proteome, the identification and the relative amount of proteins in different cellular fractions, namely, the plasmodesmata, plasma membrane, total cell extract, microsomal and cell wall fractions, were determined using label-free quantification method. Four to si...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-07-05 | PXD006800 | Pride
Arabidopsis thaliana Label-free Plasmodesmata Proteomics 
ProteomicsComparative analysis of four- and seven-day-old Arabidopsis thaliana plasmodesmata fractions using label free method
To compare four- and seven-day-old Arabidopsis thaliana plasmodesmata proteome, the identification and the relative amount of proteins in plasmodesmata, cell wall and total cell extracts were performed using fractions purified from four- and seven-day-old liquid culture cells of A. thaliana. The rel...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-07-05 | PXD006806 | Pride
Arabidopsis thaliana  plasmodesmata  proteomics  label-free 
ProteomicsProteomic analysis of plasmodesmata from Populus cell suspension cultures
In this project, we have investigated the proteome of the plasmodesmata (PD) enriched membrane fraction from poplar cell suspension cultures and quantitatively compared its protein composition with other membrane fractions. We build on the previously published literature on the PD proteome by using ...
ORGANISM(S): Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa) 
2018-12-31 | PXD010692 | Pride
Mass spectrometry Plasmodesmata Callose Populus Spectral counting Callose synthase 
ProteomicsA high confidence Physcomitrium patens plasmodesmata proteome
Cells of multicellular organisms exchange nutrients, building blocks and information. In animals this exchange happens via gap junctions, in plants via plasmodesmata (PD). PD have striking properties with a large range of molecules from ions, to metabolites and synthetic dyes, RNA and proteins up t...
ORGANISM(S): Physcomitrella patens subsp. patens (Moss) 
2024-01-18 | PXD032820 | Pride
Plasmodesmata Physcomitrium patens Proteome 
ProteomicsMultiple C2 domains and Transmembrane region Proteins (MCTPs) tether membranes at plasmodesmata
In eukaryotes, membrane contact sites (MCS) allow direct communication between organelles. Plants have evolved unique MCS, the plasmodesmata intercellular pores, which combine organelle tethering with regulation of cell-to-cell signalling, the molecular mechanisms of which remains unknown. Here,...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-07-08 | PXD013999 | Pride
Plasmodesmata Lc-ms 
ProteomicsComparative proteomics of plasmodesmata
Plants as multi cellular organisms are composed of various specialized cell types. Most of these cell types are symplasmically connected via plasmodesmata (PD) allowing exchange of nutrients and signal molecules. To date, the molecular composition of PD is not well understood. A comprehensive and co...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-09-05 | PXD006880 | Pride
Arabidopsis thaliana Pdlp; At3g15380 Cher1 Proteomics At1g22610 C2 domain Choline transporter-like 1 Plasmodesmata Mee9 Er-body At1g51570 
GenomicsAssaying effector cell-to-cell mobility in plant tissues identifies hypermobility and indirect manipulation of plasmodesmata
Assaying effector cell-to-cell mobility in plant tissues identifies hypermobility and indirect manipulation of plasmodesmata
PRJNA1043684 | ENA
TranscriptomicsAssaying effector cell-to-cell mobility in plant tissues identifies hypermobility and indirect manipulation of plasmodesmata
In plants, plasmodesmata establish cytoplasmic continuity between cells to allow for communication and resource exchange across the cell wall. While plant pathogens use plasmodesmata as a pathway for both molecular and physical invasion, the benefits of molecular invasion (cell-to-cell movement of p...
ORGANISM(S): Arabidopsis thaliana 
2023-11-27 | GSE248400 | GEO
ProteomicsComparative phyloproteomics identifies conserved plasmodesmal proteins
Plasmodesmata are cytosolic bridges, lined by the plasma membrane and traversed by endoplasmic reticulum, connecting cells and tissues, and critical for many aspects of plant biology. While plasmodesmata are notoriously difficult to extract, tissue fractionation and proteomic analyses can yield valu...
ORGANISM(S): Physcomitrella patens subsp. patens (Moss) Arabidopsis thaliana (Mouse-ear cress) 
2023-01-13 | PXD038964 | Pride
Arabidopsis Plasmodesmata Phyloproteomics Physcomitrium 
GenomicsNovel resources to investigate leaf plasmodesmata formation in C3 and C4 monocots
Novel resources to investigate leaf plasmodesmata formation in C3 and C4 monocots
PRJNA1083800 | ENA
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