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TranscriptomicsRNA-seq time-course of Calu-3 cells infected with SARS-CoV-2 early-lineage and Alpha variant viruses
Emergence of SARS-CoV-2 variants of concern (VOCs), including the globally successful Alpha (B.1.1.7) variant, suggests viral adaptation to enhance human-to-human transmission. Although much effort has focused on characterisation of spike changes, Alpha mutations outside spike likely contribute to a...
ORGANISM(S): Homo sapiens 
2021-12-31 | E-MTAB-11275 | biostudies-arrayexpress
TranscriptomicsGenotyping amplicons from SARS-CoV-2 viruses to verify the identity of PCR products
We used endpoint PCR to verify the presence of a new subgenomic RNA in SARS-CoV-2 (termed N.iORF3) and verify using nanopore sequencing that this is expressed via a newly evolved transcription regulatory sequence (TRS). We further show that this encodes a truncated C-terminal portion of Nucleocapsid...
ORGANISM(S): Severe acute respiratory syndrome-related coronavirus 
2025-01-01 | E-MTAB-14681 | biostudies-arrayexpress
TranscriptomicsLongitudinal scRNAseq profiling of memory B cells in mRNA vaccinated individuals post BA.1-breakthrough infection
Memory B cells play a fundamental role in host defenses against viruses. This dataset aimed at understanding the recruitment and remodeling of the memory B cell repertoire in the context of BA.1-breakthrough infection in BNT162b2 mRNA vaccinated individuals. All four donors enrolled in the study had...
ORGANISM(S): Homo sapiens 
2023-08-15 | E-MTAB-12651 | biostudies-arrayexpress
ProteomicsPhosphatidic acid features double membrane vesicle formation during Hepatitis C and SARS-CoV-2 infection
Double membrane vesicles (DMVs) are used as replication organelles by pathogenic RNA viruses such as hepatitis C virus (HCV) and severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Viral DMVs are morphologically analogous to DMVs formed during autophagy, and although the proteins required...
ORGANISM(S): Homo sapiens (Human) 
2022-01-24 | PXD029692 | Pride
Dmvs Lipid phosphatidic acid Sars-cov-2 Hepatitis c 
ProteomicsISGylation of the SARS-CoV-2 N protein by HERC5 impedes N oligomerization and thereby viral RNA synthesis
Interferon (IFN)-stimulated gene 15 (ISG15), a ubiquitin-like protein, is covalently conjugated to host immune proteins such as MDA5 and IRF3 in a process called ISGylation, thereby promoting type I interferon (IFN) induction to limit the replication of Severe acute respiratory syndrome coronavirus ...
ORGANISM(S): Homo sapiens (Human) Severe acute respiratory syndrome coronavirus 2 
2024-08-14 | PXD053712 | Pride
Sars-cov-2  n protein  isg15  isgylation  herc5  papain-like protease 
TranscriptomicsGenotyping amplicons from SARS-CoV-2 viruses with or without mutations to the N.iORF3 transcription regulatory sequence to determine relative viral fitness in A549-ACE2-TMPRSS2 cells
We have demonstrated that a newly evolved TRS within the Nucleocapsid gene of SARS-CoV-2 (termed N.iORF3) leads to the expression of a novel subgenomic mRNA encoding a truncated C-terminal portion of Nucleocapsid, which is an antagonist of type I interferon production. Using reverse genetics-derived...
ORGANISM(S): Severe acute respiratory syndrome-related coronavirus 
2025-01-01 | E-MTAB-14680 | biostudies-arrayexpress
UnknownSARS-CoV-2 replicon for high-throughput antiviral screening.
Not available
S-EPMC8295919 | biostudies-literature
UnknownA PCR amplicon-based SARS-CoV-2 replicon for antiviral evaluation.
Not available
S-EPMC7838314 | biostudies-literature
UnknownConstruction and characterization of two SARS-CoV-2 minigenome replicon systems.
Not available
S-EPMC9088700 | biostudies-literature
UnknownA DNA-based non-infectious replicon system to study SARS-CoV-2 RNA synthesis.
Not available
S-EPMC9424123 | biostudies-literature
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