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ProteomicsQuantification of mitochondrial translation by pulse SILAC
We developed a simple method that combined biochemical mitochondria isolation and pulse SILAC approach to monitor the mitochondrial translation. Our approach allows us to quantify 12 out of the 13 mitochondrial translation products, the highest coverage among analogous methods reported, and provide ...
ORGANISM(S): Homo Sapiens (human) 
2022-12-28 | PXD022476 | JPOST Repository
GenomicsRNA-seq using human SH-SY5Y neuroblastoma cells used for SILAC experiment
RNA-seq using human SH-SY5Y neuroblastoma cells used for SILAC experiment
PRJNA809386 | ENA
TranscriptomicsAnalysis of HeLa cells after transfection with miR-1, miR-124 and miR-181.
Analysis of HeLa cells at 24 hours after transfection with wild type miR-1, miR-124, miR-181 versus control transfected HeLa cells. Results were compared to protein down-regulation at 48 hours measured by SILAC-MS. Analysis of HeLa cells at 24 hours after transfection with wild type miR-1, miR-124,...
ORGANISM(S): Homo sapiens 
2010-05-25 | E-GEOD-11968 | biostudies-arrayexpress
ProteomicsSpatial SILAC Regorafenib Treated Bottom-up Proteomic Analysis
Developing effective model systems to evaluate new potential chemotherapeutic reagents is critical. Three-dimensional cell cultures, such as spheroids, aid in bridging the gap between commonly used monolayer cell cultures and significantly more complex and expensive animal models. Spheroid model sys...
ORGANISM(S): Homo sapiens (Human) 
2024-08-09 | PXD038958 | Pride
Spatial silac Regorafenib Spheroids 
ProteomicsImproved detection and quantitation of RNA-interactome using DIA SILAC
Detection and quantitation of the RNA-interacting proteome is commonly achieved applying SILAC labeling, following by data-dependent acquisition (DDA) of the proteomic data. However, the limited sensitivity of the DDA approach often restricts protein detection to those with higher expression in cell...
ORGANISM(S): Homo sapiens (Human) 
2023-12-19 | PXD045318 | Pride
Hek cells Dia-silac Rna-interactome 
ProteomicsImproving SILAC quantification with data independent acquisition to investigate bortezomib-induced protein degradation
Stable isotope labeling by amino acids in cell culture (SILAC) coupled to data-dependent acquisition (DDA) is a common approach to quantitative proteomics with the desirable benefit of reducing batch effects during sample processing and data acquisition. More recently, using data-independent acquisi...
ORGANISM(S): Homo Sapiens 
2022-03-29 | PXD022659 | panorama
ProteomicsIdentification of a USP9X Substrate NFX1-123 by SILAC-based Quantitative Proteomics
The deubiquitinase USP9X related with multiple diseases including neurodegeneration, epilepsy and various type of tumor, by targeting different substrates. In our study, to deep study USP9X functional candidate substrates, we performed SILAC-based quantitative proteomic to compare knockdown USP9X w...
ORGANISM(S): Homo sapiens (Human) 
2019-05-13 | PXD012871 | Pride
Usp9x nfx1-123 silac 
ProteomicsTime-resolved analysis of proteome dynamics by TMT-SILAC hyperplexing
In this project, a more efficient methodology for obtaining multi-timepoint kinetic data for proteome-wide analyses of protein turnover is developed and used to globally measure the kinetics of protein turnover in primary human fibroblasts (HCA2-hTert). This approach takes advantage of the multiplex...
ORGANISM(S): Homo sapiens (Human) 
2016-12-14 | PXD004725 | Pride
Tmt-silac Protein degrdation Tmt Proteome dynamics Silac Human primary fibroblasts Protein synthesis Hyperplexing 
ProteomicsImproved SILAC Method for Double Labeling of Bacterial Proteome
Stable isotope labeling with amino acids in cell culture (SILAC) is a robust proteomics method with the advantages of reproducibility and easy handling. This method is popular for the analysis of mammalian cells. However, amino acid conversion in bacteria decreases the labeling efficiency and quanti...
ORGANISM(S): Escherichia coli Staphylococcus aureus 
2022-02-28 | PXD011244 | Pride
Proteomics Double labeling Bacteria Silac 
ProteomicsDevelopment and evaluation of a SILAC method for proteomic analysis of rat primary microglia
Microglia play important and dynamic roles in mediating a variety of physiological and pathological processes during the development, normal function, and degeneration of the central nervous system. We report here the development of a SILAC-labeled model system suitable for mass spectrometry-based ...
ORGANISM(S): Rattus norvegicus (Rat) 
2016-04-07 | PXD002759 | Pride
Rat Silac Microglia Primary 
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