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MetabolomicsMild cold exposure mitigates ischemic heart injury through indole-3-acetamide–AHR-mediated SMAD2 proteolysis

Ambient temperature profoundly influences organismal metabolism, physiology, and disease. While cold exposure is a well-recognized risk factor for the onset of myocardial infarction (MI), its impact on the injured heart after MI remains unexplored. Here,&nb...

2026-03-03 | MTBLS13965 | MetaboLights
ProteomicsThe phosphorylation of the Smad2/3 linker region by Nemo-like kinase regulates TGF-β signaling
Smad2 and Smad3 (Smad2/3) primarily mediates the transforming growth factor-β (TGF-β) signaling that drives cell proliferation, differentiation, and migration. The dynamics of the Smad2/3 phosphorylation provides the key mechanism for regulating the TGF-β signaling pathway. Here we identified NLK as...
ORGANISM(S): Homo sapiens (Human) 
2021-09-10 | PXD023957 | Pride
Smad3 Phosphorylation Smad2 Nlk 
ProteomicsSMAD2/3 interactome in human pluripotent stem cells
This experiment was designed to determine the interactome of SMAD2/3 in human pluripotent stem cells (hPSCs). hPSCs were cultured in standard pluripotency-promoting conditions, or induced to differentiate towards the definitive endoderm lineage for 36h. Endogenous SMAD2/3 was immunoprecipitated from...
ORGANISM(S): Homo sapiens (Human) 
2018-01-04 | PXD005285 | Pride
Smad2/3 Human pluripotent stem cells 
GenomicsSmad2 and Smad3 have differential sensitivity in relaying TGF b signaling and inversely regulate early lineage specification
The transforming growth factor beta (TGFβ) related signaling is one of the most important signaling pathways regulating early developmental events. Smad2 and Smad3 are structurally similar and it is mostly considered that they are equally important in mediating TGFβ signals. Here, we show that Sma...
ORGANISM(S): Mus musculus 
2016-03-01 | E-GEOD-76557 | biostudies-arrayexpress
TranscriptomicsRNAi profiling by array of of Smad2 and Smad3 in mouse mammary epithelial cells treated with TGF-beta to induce epithelial-to-mesenchymal transition
Effects of Smad3 and Smad2 knock-down on TGFbeta-induced epithelial-to-mesenchymal transition (EMT) in mouse mammary epithelial (Nme) cells
ORGANISM(S): Mus musculus 
2009-04-01 | E-MEXP-1342 | biostudies-arrayexpress
GenomicsSmad2 and Smad3 binding sites in H345-TbRII cells
Transforming growth factor (TGF)-beta induces apoptosis of many types of cancer cells and acts as a tumor suppressor. We found lower expression of TGF-beta type II receptor (TbRII) in most of SCLC cells and tissues than in normal lung epithelial cells and normal lung tissues, respectively. In vitro ...
ORGANISM(S): Homo sapiens 
2015-09-15 | E-GEOD-63871 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse ES cells treated by varying levels of activated Smad2/3 over time in the presence of protein synthesis
The tet-on system was activated by treatment of the cells with doxycycline (Dox) in concentrations 1 microgram per milliliter. The Smad2/3 phosphorylation was inhibited by treatment of the cells with SB-431542. The TAG1 cells were treated with SB inhibitor and Dox activator and samples were analyzed...
ORGANISM(S): Mus musculus 
2009-01-01 | E-TABM-574 | biostudies-arrayexpress
GenomicsGenome-wide profiling of Smad2 and Eomesa binding in zebrafish blastulas
Study of Smad2 and Eomesa genomic binding in zebrafish blastulas, their relationship to eachother and the relaptionship between genomic binding and Ndr1 and Eomesa responsive genes as identified by microarray and RNA-seq. Replicate ChIP samples with associated input for Smad2 and Eomesa - 4x ChIP sa...
ORGANISM(S): Danio rerio 
2014-10-15 | E-GEOD-51891 | biostudies-arrayexpress
TranscriptomicsTGF-beta/Smad2/3 signaling directly regulates several miRNAs in mouse ES Cells and early embryos
Purpose: We aimed to identify miRNAs which are induced by the Activin/Nodal effectors, P-Smad2/3, in order to further our understanding of how P-Smad2/3 controls downstream gene expression in mouse ES cells to regulate crucial biological processes. Methods: We used a previously developed Tetracyclin...
ORGANISM(S): Mus musculus 
2013-02-11 | E-GEOD-39994 | biostudies-arrayexpress
GenomicsGraded Nodal/Activin Signaling Governs ES Cell Fate Decisions via Differential Recruitment of Phospho-Smad2 to Oct4 and Distinct Target Gene Subsets.
The Nodal/Activin morphogens are secreted signaling molecules that form concentration gradients during early embryogenesis providing stem cells with positional information and differentiation instructions important for embryonic patterning. The molecular basis driving stem cell interpretation of sig...
ORGANISM(S): Mus musculus 
2011-01-31 | E-GEOD-23581 | biostudies-arrayexpress
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