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ProteomicsNew label free methods for protein relative quantification applied to the investigation of animal model of Huntington Disease.
Spectral Counts approaches (SpCs) are largely employed for the comparison of protein expression profiles in label free differential proteomics applications. Similarly, to other comparative methods, also SpCs based approaches require a normalization procedure before Fold Changes (FC) calculation. Her...
ORGANISM(S): Mus musculus (Mouse) 
2020-08-18 | PXD017471 | Pride
Label free methods  spectral counts normalization  complexity-based correction factor  huntington disease mice models 
ProteomicsA normalization method to decipher quantitative proteome changes in microbial co-culture systems
The value of synthetic microbial communities in biotechnology is gaining traction due to their ability to undertake more complex metabolic tasks than monocultures. However, a thorough understanding of strain interactions, productivity and stability is often required to optimize growth and scale up c...
ORGANISM(S): Synechococcus Elongatus Pcc 7942 = Fachb-805 Azotobacter Vinelandii Dj 
2023-11-02 | PXD046627 |
ProteomicsAngiogenic and Immunologic Proteins Identified by Deep Proteomic Profiling of Human Choroidal and Retinal Vascular Endothelial Cells
PURPOSE Diseases that involve choroidal or retinal endothelial vascular cells are leading causes of vision loss: age-related macular degeneration, retinal ischemic vasculopathies and non-infectious posterior uveitis. Proteins differentially expressed by these endothelial cell populations are potenti...
ORGANISM(S): Homo sapiens (Human) 
2018-03-26 | PXD005972 | Pride
Protein expression Mudpit Tandem mass spectrometry Human Cell culture Endothelial cells Proteomics Choroid Linear ion trap Spectral counting Retina 
UnknownNew label-free methods for protein relative quantification applied to the investigation of an animal model of Huntington Disease.
Not available
S-EPMC7473538 | biostudies-literature
ProteomicsColonization state influences the hemocyte proteome in a beneficial squid-vibrio symbiosis
The squid, Euprymna scolopes, and the luminescent bacterium, Vibrio fischeri, form a highly specific beneficial light organ symbiosis. Not only does the host have to select V. fischeri from the environment, but it must also prevent subsequent colonization by non-symbiotic microorganisms. Host macrop...
ORGANISM(S): Euprymna scolopes (Hawaiian bobtail squid) 
2014-08-05 | PXD001185 | Pride
Innate immunity Hemocytes Symbiosis Vibrio fischeri Itraq Euprymna scolopes Spectral counting 
ProteomicsA method to characterize antibody selectivity immunoprecipitation, part 2
Antibodies are used in multiple cell biology applications but there are methods to assess antibody quality, riskingdata integrity and repr developed a standard operating procedure using HEK293 cells to score and antibodies for suitability in immunoprecipitation. This method, validated in in fourind...
ORGANISM(S): Homo sapiens (Human) 
2015-07-15 | PXD002534 | Pride
Mass spectrometry Immunoprecipitation Antibody selectivity and specificity 
ProteomicsA method to characterize antibody selectivity immunoprecipitation
Antibodies are used in multiple cell biology applications but there are methods to assess antibody quality, riskingdata integrity and repr developed a standard operating procedure using HEK293 cells to score and antibodies for suitability in immunoprecipitation. This method, validated in in fourind...
ORGANISM(S): Homo sapiens (Human) 
2015-07-15 | PXD002412 | Pride
Mass spectrometry Immunoprecipitation Antibody selectivity and specificity 
UnknownaLFQ: an R-package for estimating absolute protein quantities from label-free LC-MS/MS proteomics data.
Not available
S-EPMC4147881 | biostudies-literature
UnknownOptimizing differential expression analysis for proteomics data via high-performing rules and ensemble inference.
Not available
S-EPMC11082229 | biostudies-literature
ProteomicsSIRT3 interactions and mitochondrial proteome during HCMV infection
SIRT3 has been shown to inhibit HCMV infection, but the mechanism under the antiviral effect is not clear. To identify the mediator of SIRT3 antiviral function, we aim to identify and quantify SIRT3 interactions during HCMV infection. A set of large-scale IPs were performed to determine the specific...
ORGANISM(S): Homo sapiens (Human) 
2021-04-01 | PXD018995 | Pride
Hcmv Mitochondrial proteome Ip-ms Sirt3 
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