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TranscriptomicsThe DSIF subunits Spt4 and Spt5 have several unexpected, distinct roles in regulating immunoglobulin class switch recombination
Class-switch recombination (CSR), induced by activation-induced cytidine deaminase, can be divided into two phases: DNA cleavage of the switch (S) regions, and the joining of the cleaved ends of the different S regions. Here, we show that the DSIF complex (Spt4 and Spt5), a transcription elongation ...
ORGANISM(S): Mus musculus 
2013-10-22 | E-GEOD-33206 | biostudies-arrayexpress
ProteomicsDSIF factor Spt5 coordinates transcription, maturation and exoribonucleolysis of RNA polymerase II transcripts
The timely termination of RNA polymerase II (Pol II) transcription is essential for recycling of this enzyme and preventing transcriptional interference. While 5'-3' exonuclease Xrn2 is known to degrade nascent RNA after poly(A) site cleavage to promote Pol II release, its molecular mechanism remain...
ORGANISM(S): Schizosaccharomyces pombe 
2024-11-22 | PXD058168 | Pride
Xrn2 Ms-ms Spt5 Torpedo Fission yeast (schizosaccharomyces pombe) Dsif Crosslinking 
GenomicsGenome wide localization of Activation induced cytidine deaminase (AID), Spt5, and RNA Polymerase II (PolII) in mouse
Activation induced cytidine deaminase (AID) initiates antibody gene diversification by creating U:G mismatches. However, AID is not specific for antibody genes. Off-target lesions can activate oncogenes or cause chromosome translocations. Despite its importance in these key transactions little is kn...
ORGANISM(S): Mus musculus 
2010-10-05 | E-GEOD-24178 | biostudies-arrayexpress
GenomicsIntegrator complex regulates NELF-mediated RNA Polymerase II pause/release and processivity at coding genes [ChIP-seq]
RNAPII pausing/termination shortly after initiation is a hallmark of gene regulation. However, the molecular mechanisms involved are still to be uncovered. Here, we show that NELF interacts with Integrator complex subunits (INTScom) forming a stable complex with RNPII and Spt5. The interaction betwe...
ORGANISM(S): Homo sapiens 
2015-05-19 | E-GEOD-60584 | biostudies-arrayexpress
TranscriptomicssiRNA profiling of Zebrafish embryos knocked down for Spt5
The Spt4-Spt5 complex, and its human homolog DSIF (DRB sensitivity-inducing factor), is unique in its ability to regulate Pol II processivity. Previous studies have shown that Spt5 has the characteristics of a general transcription-elongation factor. However, mutagenesis of Spt5 showed specific phen...
ORGANISM(S): Danio rerio 
2008-06-14 | E-GEOD-6127 | biostudies-arrayexpress
GenomicsTotal RNA Pol2 ChIP-seq in mES cells treated with flavopiridol (Cdk9 inhibitor) after DSIF and NELF knockdown
Recent studies have found that promoter-proximal pausing occurs at most Pol2-regulated genes. DSIF and NELF function as negative elongation factors and promote Pol2 pausing. P-TEFb, whose enzymatic activity lies in the kinase Cdk9, positively regulates the transition into productive elongation by ...
ORGANISM(S): Mus musculus 
2011-01-19 | E-GEOD-26658 | biostudies-arrayexpress
GenomicsLEDGF/p75 promotes transcriptional pausing through preventing SPT5 phosphorylation
LEDGF/p75 promotes transcriptional pausing through preventing SPT5 phosphorylation
PRJNA1053782 | ENA
UnknownDual roles for Spt5 in pre-mRNA processing and transcription elongation revealed by identification of Spt5-associated proteins.
Not available
S-EPMC141151 | biostudies-literature
ProteomicsThe transient Spt4-Spt5 complex as an upstream regulator of non-coding RNAs during development
The Spt4-Spt5 complex is conserved and essential RNA polymerase elongation factor. To investigate the role of the Spt4-Spt5 complex in non-coding transcription during development, we used the unicellular model Paramecium tetraurelia. In this organism harboring both germline (micronucleus - MIC) and ...
ORGANISM(S): Paramecium tetraurelia 
2022-04-27 | PXD027748 | Pride
Interactomics Spt4 Meiosis 
UnknownThe pleiotropic roles of SPT5 in transcription.
Not available
S-EPMC9467590 | biostudies-literature
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