Sort   by:  
 Page size 
MetabolomicsThe in-gel proteomic characterization of exercise-induced sweat - (part III)
Not available
ORGANISM(S): Homo sapiens 
ST001072 | MetabolomicsWorkbench
ProteomicsAnalysis of proteins in supernatant and precipitate fractions of human bronchial smooth muscle cells by 1D SDS-PAGE and quantitative LC-MS/MS
Supernatant and precipitate protein fractions of human bronchial smooth muscle cells (HBSMC) after sonication and centrifugation were analyzed using 1D SDS-PAGE. The gel lanes were cube-cut into thirty-three 1.1 mm x 1.1 mm x 1 mm (gel thickness) squares and each gel piece was subjected to in-gel d...
ORGANISM(S): Homo sapiens (Human) 
2019-01-03 | PXD010036 | Pride
Precipitation fraction 1d sds-page Supernatant fraction Bronchial smooth muscle cells Quantitative lc-ms/ms 
ProteomicsMore is not always better: Evaluation of 1D- and 2D-LC-MS2 methods for metaproteomics
Metaproteomics is a powerful tool to characterize the structure of microbial communities and the physiology, metabolism and interactions of the species in these communities. Metaproteomics seeks to identify and quantify proteins from microbial communities on a large scale using gel electrophoresis o...
ORGANISM(S): Eukaryota (eucaryotes) Bacteria Archaea Viruses 
2019-02-12 | PXD008017 | Pride
Microbial community  microbiota  microbiome  mock community  method evaluation  microbial ecology  q exactive  liquid chromatography  gelc 
ProteomicsTAP-ERK2-K52R MS analysis of 1D gel bands
MS analysis of 1D gel bands from TAP-ERK2-K52R immunoprecipitation experiments
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000088047 | MassIVE
ProteomicsComparative Two-Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) of Human Milk to Identify Dysregulated Proteins in Breast Cancer
Breast Cancer (BC) remains a major cause of mortality, and early detection is considered important for reducing BC-associated deaths. Early detection of BC is challenging in young women, due to the limitations of mammography on the dense breast tissue of young women. We recently reported results of ...
ORGANISM(S): Homo sapiens (Human) 
2018-06-25 | PXD009860 | Pride
Mass spectrometry Breast cancer Breast milk 2d-page Proteomics Biomarkers 
ProteomicsA new method for differential proteomic analysis utilizing native MS-electropherograms: a comparison between plasma and serum proteins by combining native PAGE, whole-gel slicing and quantitative LC-MS/MS
MS identification has long been used for PAGE-separated protein bands, but global and systematic quantitation utilizing MS after PAGE has remained rare and not been reported for native PAGE. Here we reported on a new method combining native PAGE, whole-gel slicing and quantitative LC-MS/MS aiming c...
ORGANISM(S): Homo sapiens (Human) 
2017-09-08 | PXD006773 | Pride
Gel slicing Quantitative lc-ms/ms Native ms-electropherogram Native 1d-page Human plasma and serum Differential proteomic analysis 
UnknownAutomatic DNA Diagnosis for 1D Gel Electrophoresis Images using Bio-image Processing Technique.
Not available
S-EPMC4682448 | biostudies-literature
Proteomics1DE-MS profiling of rat cerebral cortex proteins of focal cerebral ischemia-reperfusion injury by combining SDS-PAGE, gel slicing, quantitative LC-MS/MS and profile reconstruction
A rat model of focal cerebral ischemia-reperfusion (I/R) injury was established by 2 hour-middle cerebral artery occlusion (MCAO) followed with 1, 7 and 14 days of reperfusion. Validation of the model was done by the measurement on cerebral blood flow, infarct volume, histological damage and neurol...
ORGANISM(S): Rattus Norvegicus 
2022-05-11 | PXD033793 |
ProteomicsIdentification of LMTK3 protein by LC/MS/MS Analysis of 1D SDS PAGE Gel Bands
Identification of LMTK3 by LC/MS/MS. A plasmid encoding for full-length LMTK3 was overexpressed in MCF-7 breast cancer cells. Following antibody detection by WB, two bands of interest were seen at around 200 kDa and 150 kDa on the blotting membrane. Colloidal Coomassie (CCBB) staining was performed ...
ORGANISM(S): Homo sapiens (Human) 
2022-02-22 | PXD001004 | Pride
Silac Lmtk3 Breast cancer Integrin b1 Grb2 
ProteomicsMembrane proteins of Planctopirus limnophila
Bacteria of the phylum Planctomycetes have been postulated to be the ‘missing link’ between prokaryotes and eukaryotes. Although phylogenetically the Planctomycetes were grouped into Gram-negative bacteria, it has been reported that they have no outer membrane. Here we present 1D-gel-LC/MS/MS based...
ORGANISM(S): Planctopirus limnophila DSM 3776 
2017-04-18 | PXD005738 | Pride
Planctomycetes 1d-gel-lc-ms/ms Membrane proteins 
Sort   by:  
 Page size