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ProteomicsGlobal mapping of rat plasma proteins with a native proteomic approach using nondenaturing micro 2DE and label-free quantitative LC-MS/MS
Plasma samples from adult male rats were separated by nondenaturing micro 2DE and a representative gel was selected, on which a total of 136 CBB-stained spots were numbered and subjected to in-gel digestion and label-free quantitative LC-MSE (MS/MS in data-independent acquisition mode). The analysi...
ORGANISM(S): Rattus norvegicus (Rat) 
2016-10-14 | PXD004395 | Pride
Native proteomics Adult male rat plasma proteome Nondenaturing micro 2de Global mapping Label-free quantitative lc-mse 
ProteomicsUpdate on virtual-experimental 2DE approach in chromosome-centric human proteome project
In the boundaries of chromosome-centric Human Proteome Project c-HPP to obtain information about proteoforms coded by chromosome 18, several cell lines were analyzed. In our study we have been using proteoform separation by 2DE (sectional analysis) and semi-virtual 2DE with following shotgun mass-sp...
ORGANISM(S): Homo sapiens (Human) 
2018-09-24 | PXD010142 | Pride
Esi lc-ms/ms Inventory Proteoforms Mass spectrometry Chromosome 18 Chromosome-centric Two-dimensional electrophoresis Proteome 
ProteomicsProteomic analysis of cellular soluble proteins from human bronchial smooth muscle cells by combining nondenaturing micro 2DE and quantitative LC-MS/MS: Preparation of more than 4000 native protein maps
Soluble proteins of human bronchial smooth muscle cells (HBSMC) were separated by nondenaturing micro 2DE and a 30 mm X 40 mm area of the CBB-stained slab gel (1.0 mm thick) was cut into 1.1 mm X 1.1 mm squares, then the proteins in the 972 gel pieces (squares) were applied to quantitative LC-MS/MS....
ORGANISM(S): Homo sapiens (Human) 
2015-08-10 | PXD001471 | Pride
Cellular proteins Native protein map Nondenaturing micro 2de Lc-ms/ms Quantitative protein-protein interaction 
ProteomicsUpdate on virtual-experimental 2DE approach in chromosome-centric human proteome project
In the boundaries of chromosome-centric Human Proteome Project c-HPP to obtain information about proteoforms coded by chromosome 18, several cell lines were analyzed. In our study we have been using proteoform separation by 2DE (sectional analysis) and semi-virtual 2DE with following shotgun mass-sp...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-11-12 | MSV000086448 | MassIVE
TranscriptomicsMolecular variability of FLT3/ITD mutants and their impact on the differentiation program of 32D cells
FLT3 is the most frequently mutated gene in AML – up to 40% of AML harbor an activating mutation within FLT3 gene. Though AML is a relatively rare disease, such a high mutability rate, as observed with FLT3 gene, is striking. To elucidate the molecular background of this phenomenon, we have establ...
ORGANISM(S): Mus musculus 
2010-04-08 | E-GEOD-12766 | biostudies-arrayexpress
ProteomicsAQP8 knockout project - 2DE of wild and AQP8 knockout mice colon
2DE of wild and AQP8 knockout mice colon
ORGANISM(S): Mus musculus wagneri 
2010-07-01 | PRD000252 | Pride
ProteomicsProteomic profiling of HepG2 cell line after 2DE fractionation
One of the major goals of the Chromosome-centric Human Proteome Project (C-HPP) is to catalog and annotate myriads of heterogeneous proteoforms, produced by ca. 20 thousand genes. To achieve deeper and personalized dive into proteome we suggest to use customized RNA-seq library of potential proteofo...
ORGANISM(S): Homo sapiens (Human) 
2020-06-01 | PXD018450 | Pride
Proteoforms Hepg2 Lc-ms/ms 
UnknownConstruction of 2DE Patterns of Plasma Proteins: Aspect of Potential Tumor Markers.
Not available
S-EPMC9569744 | biostudies-literature
UnknownFollowing up of Surgical Treated Human Liver Cystic Echinococcosis: A Proteomics Approach.
Not available
S-EPMC7988678 | biostudies-literature
UnknownEvaluation of three high abundance protein depletion kits for umbilical cord serum proteomics.
Not available
S-EPMC3105942 | biostudies-literature
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