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TranscriptomicsGenomic characterization of ovarian cancer spheroids
Spheroids are 3D multi-cell aggregates formed in non-addherent culture conditions. In ovarian cancer (OC), they serve as a vehicle for cancer cell dissemination in the peritoneal cavity. We investigated genes and networks upregulated in three dimensional (3D) versus two-dimensional (2D) culture cond...
ORGANISM(S): Homo sapiens 
2014-01-03 | E-GEOD-53759 | biostudies-arrayexpress
ProteomicsAcetaminophen-induced S-nitrosylation and S-sulfenylation signalling in 3D cultured hepatocarcinoma cell spheroids.
In this project we have studied human hepatoma cell line grown in 3D spheroids subjected to APAP (acetaminophen) treatment. Global protein expression analysis (label free) and analysis of cysteine oxidations (SNO/SOH TMT proteomics) have been combined to get insights in to the molecular response tri...
ORGANISM(S): Homo sapiens (Human) 
2018-10-18 | PXD003587 | Pride
Apap  3d cell culture  s-nitrosylation  s-sulfenylation  quantitative redox proteomics  toxicology  hepg2/c3a 
ProteomicsOCT1 (SLC22A1) transporter kinetics and regulation in primary human hepatocyte 3D spheroids
3D spheroids of primary human hepatocytes (3D PHH) keep a differentiated phenotype with retained metabolic function and proteome fingerprint for weeks in culture. As a result, 3D PHH are gaining ground as a model for mechanistic studies on liver homeostasis and in vitro to in vivo (IVIV)predictions...
ORGANISM(S): Homo sapiens (Human) 
2024-08-10 | PXD045733 | Pride
Human Hepatocytes Adme Liver 
ProteomicsGlobal proteomic and phosphoproteomic analysis of WT HEC-50B and MAPK14-/- HEC-50B cells cultured as 2D monolayers and 3D spheroids.
The molecular underpinnings of High Grade Endometrial Carcinoma (HGEC) metastatic growth and survival are poorly understood. Here we show that ascites-derived and primary tumor HGEC cell lines in 3D spheroid culture faithfully recapitulate key features of malignant peritoneal effusion and exhibit fu...
ORGANISM(S): Homo sapiens (Human) 
2025-10-07 | PXD053011 | Pride
Endometrial cancer P38a Mapk14 3d spheroids 
ProteomicsSmooth muscle cell spheroids as 3D model of phenotypic plasticity and matrix deposition revealed by 2D-3D proteomics
Our study aims to compare 2D adherent and 3D floating (spheroid) cell cultures of in the context of cellular physiology and in atherosclerosis modelling. Here, we utilize vascular smooth muscle cells from rat aorta to profile 2D and 3D proteomes.
ORGANISM(S): Rattus norvegicus (Rat) 
2026-09-07 | PXD069144 | Pride
Smoot muscle cell  spheroids  3d cell culture  lc-ms/ms 
ProteomicsDiesel particulate matter-induced proteomic changes in three-dimensional spheroids derived from human primary cells of various tissue origins
This study investigated the effects of diesel particulate matter (DPM) on multiple human organs using 3D spheroids derived from eight human primary cell types. To assess the impact of DPM, we exposed these spheroids to varying concentrations of standardized DPM (SRM 2975) and measured their viabilit...
ORGANISM(S): Homo sapiens (Human) 
2025-08-21 | PXD056979 | Pride
Three-dimensional (3d) spheroids Mass spectrometry (ms) Diesel particulate matter (dpm) Viability assessment Tandem mass tag (tmt) 
ProteomicsCarnosine effect in scaffold free human dermis spheroids model
Carnosine is an endogenous β-alanyl-L-histidine dipeptide endowed of antioxidant and carbonyl scavenger properties, able to significantly prevent the visible signs of aging and photoaging. To deeply investigate the mechanism of action of carnosine on human skin proteome, a 3D scaf-fold-free spheroid...
ORGANISM(S): Homo sapiens (Human) 
2022-02-22 | PXD031053 | Pride
Carnosine Spheroids Label free 
ProteomicsHepatocyte thorns, a novel drug-induced stress response in human and mouse liver spheroids
The in vivo-relevant phenotype of 3D liver spheroids allows for long-term studies of e.g. novel mechanisms of chronic drug-induced liver toxicity. Using this system, we present a novel drug-induced stress response in human and murine hepatocyte spheroids wherein long slender filaments form after chr...
ORGANISM(S): Homo sapiens (Human) 
2022-05-26 | PXD033550 | Pride
Hepatocytes  spheroids  thorns  hepatotoxicity  keratins  3d culture 
ProteomicsAnimal product-free culture conditions for 3D primary human hepatocyte spheroids improve long-term viability and drug metabolism
3D cultures of primary human hepatocytes (3D PHH) are successfully used to reduce and replace the use of animal experiments in biomedical research. Yet, the initial formation of 3D PHH is highly dependent on the supplementation with fetal bovine serum (FBS). However, the molecular composition of FBS...
ORGANISM(S): Homo sapiens (Human) 
2026-01-23 | PXD056428 | Pride
Human Setum-free Fbs Liver 3d phh 
GenomicsTranscriptomic profile of 3D spheroids developed from Astyanax Liver-derived cells - SFL and CFL.
Transcriptomic profile of 3D spheroids developed from Astyanax Liver-derived cells - SFL and CFL.
PRJNA980547 | ENA
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