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ProteomicsAMPylation Regulates 5'-3' Exonuclease PLD3 processing
The 5’-3’ exonuclease phospholipase D3 (PLD3) is a type II transmembrane protein undergoing sequential post-translational modifications (PTM) by N-glycosylation, AMPylation and proteolytic cleavage. The substrates of PLD3 5’-3’ exonuclease activity are single-stranded DNAs and RNAs, which act as lig...
ORGANISM(S): Homo sapiens (Human) 
2025-09-11 | PXD057707 | Pride
Pld3 Ampylation Chemical proteomics Protein post-translational modifications 
TranscriptomicsNascent strand with BrdU or lambda exonuclease
Nascent strand were purified with either the BrdU or the lambda exonuclease methods from culture human basophilic erythroblasts and sequenced/ Two methods were compared to isolated the Nascent strand, lambda exonuclease digestion or immunoprecipitation after pulse labelling with BrdU The following...
ORGANISM(S): Homo sapiens 
2014-05-01 | E-GEOD-50976 | biostudies-arrayexpress
TranscriptomicsAnalysis to evaluate the presence of 5’ cap in intragenic lncRNAs
RNA aliquots were digested with 5’-phosphate-dependent exonuclease (5'-exo) following pre-treatment with tobacco acid pyrophosphatase (TAP) to release the 5’ cap and render the RNA susceptible to 5’ exonuclease digestion (TAP+/5'-exo+). Two color array hybridizations were performed and TAP+/5'-exo+...
ORGANISM(S): Homo sapiens 
2015-06-30 | E-GEOD-62984 | biostudies-arrayexpress
MetabolomicsPositional Enrichment by Proton Analysis (PEPA): a One-Dimensional 1H-NMR Approach for 13C Stable Isotope Tracer Studies in Metabolomics
A novel metabolomics approach for NMR-based stable isotope tracer studies called PEPA is presented, and its performance validated using human cancer cells. PEPA detects the position of carbon label in isotopically enriched metabolites and quantifies fractional enrichment by indirect determination of...
2017-02-23 | MTBLS247 | MetaboLights
GenomicsTranscriptome-Wide Analysis of the 5' Cap Status of RNA Using 5' Monophosphate-Dependent Exonuclease Digestion and RNA Sequencing
Transcriptome-Wide Analysis of the 5' Cap Status of RNA Using 5' Monophosphate-Dependent Exonuclease Digestion and RNA Sequencing
PRJNA839349 | ENA
UnknownThe hSNM1 protein is a DNA 5'-exonuclease.
Not available
S-EPMC2094091 | biostudies-literature
TranscriptomicsA Tetrahymena Piwi protein and the exonuclease Xrn2 form a complex that is essential for ribosome biogenesis
Emerging evidence suggests that Ago/Piwi proteins function in the nucleus as well as the cytoplasm to control gene expression, but the mechanisms they employ in the nucleus remain poorly defined. The Tetrahymena thermophila Ago/Piwi protein Twi12 is essential for growth and functions in the nucleus....
ORGANISM(S): Tetrahymena thermophila 
2012-08-31 | E-GEOD-38507 | biostudies-arrayexpress
TranscriptomicsRNA-seq analysis of IMQ-treated wt and Trex2-/- skin
TREX2 is a keratinocyte specific 3’-deoxyribonuclease that participates in the maintenance of skin homeostasis upon damage. This transcriptome analysis identified multiple genes and pathways deregulated by TREX2 loss in the IMQ-induced psoriasis-like model in mouse skin. mRNA sequencing of 5 biolo...
ORGANISM(S): Mus musculus 
2016-09-07 | E-GEOD-74528 | biostudies-arrayexpress
ProteomicsStructural analysis of PLD3 reveals insights into the mechanism of lysosomal 5' exonuclease-mediated nucleic acid degradation
The phospholipase D (PLD) family is comprised of enzymes bearing phospholipase activity towards lipids or endo- and exonuclease activity towards nucleic acids. PLD3 is synthesized as a type II transmembrane protein and proteolytically cleaved in lysosomes yielding a soluble active form. The deficien...
ORGANISM(S): Mus musculus (Mouse) 
2023-11-24 | PXD044504 | Pride
Lysosome Phospholipase d Structural biology Dna/rna degradation 5' exonuclease Pld3 
UnknownMetastasis suppressor function of NM23-H1 requires its 3'-5' exonuclease activity.
Not available
S-EPMC2946830 | biostudies-literature
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