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ProteomicsXL-MS of 5S RNP preribosomal complexes
The 5S RNP is assembled from its three components (5S rRNA, Rpl5/uL18, and Rpl11/uL5) before being incorporated into the pre-60S subunit. However, when ribosome synthesis is disturbed, a free 5S RNP can enter the MDM2–p53 pathway to regulate cell cycle and apoptotic signaling. Here we reconstitute a...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-06-13 | PXD040087 | Pride
5s rnp Cryo-em Mdm2 
ProteomicsDepletion and reconstitution of 5S RNP complexes in early pre60S ribosomes
We purified pre60S ribosomes from yeast using Nsa1-FtpA as bait in Tandem Affinity Purification experiments. We tested the depletion of RPF2, using a Galactose-depletion system, to obtain ribosomal particles depleted of 5S RNP complexes. Sample A1: Control group (wild type strain) grown in YPGal; Sa...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-10-24 | PXD040306 | Pride
5s rnp Ribosomes Reconstitution Depletion Pre60s 
Proteomics• SURF2 is a new key player in nucleolar stress response through the regulation of 5S RNP/MDM2/p53 axis.
Cancer cells are addicted to strong ribosome production to sustain their proliferation rate. Many chemotherapies impede ribosome production which is perceived by cells as "nucleolar stress" (NS), triggering TP53-dependent and independent response pathways leading to cell cycle arrest and/or apoptosi...
ORGANISM(S): Homo sapiens (Human) 
2024-09-30 | PXD047905 | Pride
Cancer Ribosome Nucleolar stress Human 
UnknownThe 5S RNP couples p53 homeostasis to ribosome biogenesis and nucleolar stress.
Not available
S-EPMC3808153 | biostudies-literature
OtherChaperoning 5S RNA assembly by structural probing
In eukaryotes, three of the four ribosomal RNAs (rRNAs), the 5.8S, 18S and 25S/28S rRNAs, are processed from a single pre-rRNA transcript and assembled into ribosomes. The fourth rRNA, the 5S rRNA, is transcribed by RNA polymerase III and is assembled into the 5S ribonucleoprotein particle (5S RNP),...
ORGANISM(S): Saccharomyces cerevisiae BY4741 
2015-07-20 | GSE68431 | GEO
UnknownSymportin 1 chaperones 5S RNP assembly during ribosome biogenesis by occupying an essential rRNA-binding site.
Not available
S-EPMC4396368 | biostudies-literature
UnknownNon-canonical binding interactions of the RNA recognition motif (RRM) domains of P34 protein modulate binding within the 5S ribonucleoprotein particle (5S RNP).
Not available
S-EPMC5436847 | biostudies-literature
UnknownSuppressor mutations in Rpf2-Rrs1 or Rpl5 bypass the Cgr1 function for pre-ribosomal 5S RNP-rotation.
Not available
S-EPMC6173701 | biostudies-literature
UnknownMechanism of 5S RNP recruitment and helicase-surveilled rRNA maturation during pre-60S biogenesis.
Not available
S-EPMC10328080 | biostudies-literature
OtherSaturation mutagenesis of the human 5S rRNA gene defines expression- and ribosome-incorporation-defective variants
The transcribed 5S rRNA gene of the human 5S rDNA array was saturation-mutagenised in an expression plasmid and transfected into HEK293T cells. Cells were fractionated on sucrose gradients and 5S rRNA sequenced from the input (total) RNA and the assembled 60S large ribosomal subunit, alongside the i...
ORGANISM(S): Homo sapiens 
2026-09-02 | GSE339543 | GEO
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