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ProteomicsA Chemical Acetylation based Mass Spectrometry Platform for Histone Profiling
Here we present a strategy for the identification and quantitation of histone methylation that utilizes nondeuterated acetic anhydride to fully acetylate all free lysine residues prior to digestion with trypsin and analysis by LC-MS/MS.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000086146 | MassIVE
GenomicsHistone H3K4 Demethylation is Negatively Regulated by Histone H3 Acetylation in S. cerevisiae
Histone H3 lysine 4 tri-methylation (H3K4me3) is a hallmark of transcription initiation, but how H3K4me3 is demethylated during gene repression is poorly understood. Jhd2, a JmjC domain protein, was recently identified as the major H3K4me3 histone demethylase (HDM) in S. cerevisiae. While JHD2 is ...
ORGANISM(S): Saccharomyces cerevisiae 
2012-11-05 | E-GEOD-41424 | biostudies-arrayexpress
ProteomicsInfluence of digestion protocol in Protein Quantitation
Quantitative determination of absolute and relative protein amounts is an essential requirement for most current bottom-up proteomics applications, but protein quantitation estimates are affected by several sources of variability such as sample preparation, mass spectrometric acquisition, and data a...
ORGANISM(S): Escherichia coli 
2014-07-30 | PXD001187 | Pride
Protein quantitation Digestion protocols Fasp 
ProteomicsGlobal acetylation profiling for multiple Arabidopsis thaliana plastid N-terminal acetyltransferases
Protein acetylation is a universally conserved modification occurring on N-termini (N-Terminal acetylation, NTA). Although recent reports indicate that NTA occur frequently in plant plastids, little is known about the machinery involved in plastid acetylation and why these modifications are that fre...
ORGANISM(S): Escherichia coli 
2020-07-13 | PXD016205 | Pride
Acetyltransferase N-acetylome N-terminal acetylation Quantitative proteomics Silpronaq Gap test 
ProteomicsQuantifying Protein Acetylation in Diabetic Nephropathy from FFPE Explants
Background: Diabetic kidney disease (DKD) is a serious complication of diabetes mellitus and a leading cause of chronic kidney disease and end stage renal disease. One potential mechanism underlying cellular dysfunction contributing to kidney disease is aberrant post-translational modifications, suc...
ORGANISM(S): Homo sapiens (Human) 
2025-11-10 | PXD041884 | Pride
Proteomics Formalin-fixed paraffin-embedded tissue Mass spectrometry Kidney disease Acetylation Diabetes 
ProteomicsN-terminal acetylation by NatB in Arabidopsis thaliana
N-terminal acetylation (NTA) is one of the most abundant protein modifications in eukaryotes and is catalysed in humans by seven N-acetyltransferases. In this study, we investigated the Arabidopsis thaliana NatB catalytic (NAA20) and auxiliary subunit (NAA25) and their influence on protein N-termina...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-11-21 | PXD016209 | Pride
Acetyltransferase N-acetylome N-terminal acetylation Quantitative proteomics Silpronaq Gap test 
ProteomicsTargeted Protein Acetylation in Cells Using Heterobifunctional Molecules
Protein acetylation is a central event in orchestrating diverse cellular processes. However, current strategies to investigate protein acetylation in cells are often non-specific or lack temporal and magnitude control. Here, we developed an acetylation tagging system, AceTAG, to induce acetylation o...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD027617 | Pride
Targeted acetylation Acetylproteomics P300/cbp Heterobifunctional molecules 
ProteomicsLipids reprogram cellular metabolism to become a major carbon source for histone acetylation.
From McDonnell et. al. Cell Reports 2016: Cells integrate nutrient sensing and metabolism to coordinate proper cellular responses to a particular nutrient source. For example, glucose drives a gene expression program characterized by activating genes involved in its metabolism, in part, by increasi...
ORGANISM(S): Mus musculus (Mouse) 
2016-12-22 | PXD005040 | Pride
Lipid Proteomics Histone acetylation 
ProteomicsGlobal acetylation profiling for the Arabidopsis thaliana plasma membrane anchored NAA60 N-terminal acetyltransferase
N-terminal acetylation is one of the most abundant protein modifications in eukaryotes and is catalysed in humans by seven N-terminal acetyltransferases. AtNAA60 is localized in vivo at the plasma membrane by an α-helical membrane anchor at its C-terminus. In this study, we investigated the Arabidop...
ORGANISM(S): Escherichia coli 
2020-07-15 | PXD016533 | Pride
Acetyltransferase N-acetylome N-terminal acetylation Quantitative proteomics Silpronaq Gap test 
ProteomicsGlobal acetylation profiling for the Arabidopsis thaliana the N-acetyltransferase NAA50 in the NatA complex.
N-terminal acetylation is one of the most abundant protein modifications in eukaryotes and is catalysed in humans by seven N-terminal acetyltransferases. AtNAA50 interreact with the NatA complex (NAA10 and NAA15) to form the NatE complex. In this study, we investigated the activity of this catalytic...
ORGANISM(S): Escherichia coli 
2020-07-15 | PXD017767 | Pride
Acetyltransferase N-acetylome N-terminal acetylation Quantitative proteomics Silpronaq Gap test Encounter 
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