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ProteomicsEngineering Af1521 macro domain increases ADP-ribose binding affinity and leads to the identification of more ADP-ribosylated proteins
Protein ADP-ribosylation is a covalent, reversible post-translational modification that has important functions in several cellular mechanisms. The identification of modified proteins in cells has proven challenging and, due to the low abundance of protein ADP-ribosylation, has mainly been possible ...
ORGANISM(S): Homo sapiens (Human) 
2020-10-20 | PXD016686 | Pride
Ap-ms Af1521 macro domain Adp-ribosylation Lc-ms/ms 
UnknownEngineering Af1521 improves ADP-ribose binding and identification of ADP-ribosylated proteins.
Not available
S-EPMC7566600 | biostudies-literature
UnknownDetection of ADP-Ribosylation of the Androgen Receptor Using the Recombinant Macrodomain AF1521 from Archaeoglobus fulgidus.
Not available
S-EPMC7825248 | biostudies-literature
ProteomicsAffinity purification of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effector AvrRpm1 (2.5 ug Af1521 Macro, technical replicate 1)
ADP-ribosylated proteins were enriched using the engineered Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w) from transgenic A. thaliana plants expressing the Pseudomonas syringae type III effector AvrRpm1. Enriched proteins were analyzed by LC-MS/MS.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-09-28 | PXD045544 | Pride
Arabidopsis thaliana Type iii effector Pseudomonas syringae Plant pathogen Adp-ribosylation 
ProteomicsAffinity purification of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effector AvrRpm1 (5 ug Af1521 Macro, technical replicate 2)
ADP-ribosylated proteins were enriched using the engineered Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w) from transgenic A. thaliana plants expressing the Pseudomonas syringae type III effector AvrRpm1. Enriched proteins were analyzed by LC-MS/MS.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-09-28 | PXD045545 | Pride
Arabidopsis thaliana Type iii effector Pseudomonas syringae Plant pathogen Adp-ribosylation 
ProteomicsAffinity purification of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effector AvrRpm1, DDA method (2.5 ug Af1521 Macro, technical replicate 1)
ADP-ribosylated proteins were enriched using the engineered Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w) from transgenic A. thaliana plants expressing the Pseudomonas syringae type III effector AvrRpm1. Enriched proteins were analyzed by LC-MS/MS.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-09-28 | PXD045558 | Pride
Arabidopsis thaliana Type iii effector Pseudomonas syringae Plant pathogen Adp-ribosylation 
ProteomicsAffinity purification of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effector AvrRpm1, DDA method (5 ug Af1521 Macro, technical replicate 2)
ADP-ribosylated proteins were enriched using the engineered Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w) from transgenic A. thaliana lines expressing the Pseudomonas syringae type III effector AvrRpm1. Enriched proteins were analyzed by LC-MS/MS.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-09-28 | PXD045548 | Pride
Arabidopsis thaliana Type iii effector Pseudomonas syringae Plant pathogen Adp-ribosylation 
ProteomicsSupplementary Dataset S7. ADP-ribosylated peptides identified in two Af1521 Macro pulldown experiments from HopU1-Flag vs. Col-0 samples by the ‘triggering’ method.
During infection, the plant pathogenic bacterium Pseudomonas syringae translocates type 3 effector proteins into the host cell. The effector protein HopU1 is an ADP-ribosyltransferases that ADP-ribosylates host proteins to subvert plant innate immunity. The aim of this experiment was to compare affi...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-11-17 | PXD068739 | Pride
Hopu1 Lc-msms Arabidopsis thaliana Affinity purification Plant Pseudomonas syringae Adp-ribosylation Plant pathogen Type 3 effector 
ProteomicsDeciphering cytokine-driven ADP-ribosylation signaling networks via Af1521-based mass spectrometry analysis of labile Glu/Asp-linkages
ADP-ribosylation (ADPr) is a regulatory post-translational modification targeting nine amino acid residues, but glutamate/aspartate-linked ADPr (Glu/Asp-ADPr) is labile and remains challenging to detect using conventional mass spectrometry (MS)-based workflows. Using synthetic peptides, we show that...
ORGANISM(S): Homo sapiens (Human) 
2026-05-27 | PXD070310 | Pride
ProteomicsSupplementary Dataset S4. Comparison of proteins identified in three experiments using the functional (Af1521) versus non-functional (G42E) Macro domains following ADPr-enrichment of proteins from Dex-treated AvrRpm1-HA plants.
During infection, the plant pathogenic bacterium Pseudomonas syringae translocates type 3 effector proteins into the host cell. The effector protein AvrRpm1 is an ADP-ribosyltransferases that ADP-ribosylates host proteins to subvert plant innate immunity. The aim of this experiment was to compare af...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-11-17 | PXD068551 | Pride
Lc-msms Arabidopsis thaliana Affinity purification Plant Pseudomonas syringae Adp-ribosylation Avrrpm1 Plant pathogen Type 3 effector 
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