PKR-like kinase (PERK) plays a significant role in inducing angiogenesis in various cancer-types including glioblastoma. Mass spectrometry screen on conditioned media from LN308 glioblastoma cell line treated with hypoxia revealed Peptidylglycine α-amidating monooxygenase (PAM) as a potential target...
Synaptosome (Syn) and synaptic junctions (SJ) are widely used in omics experiments to investigate protein content and networks of synapses. Synapses are a neuronal compartment wit a high lipid content. Liquid-liquid extraction (LLE), typically used in lipidomics, shows great potential for multiomics...
Membrane lipids and their metabolism have key functions in neurotransmission. Here we provide a quantitative lipid inventory of mouse and rat synaptic junctions. To this end, we developed a multiomics extraction and analysis workflow to probe the interplay of proteins and lipids in synaptic signal t...
Saccharomyces cerevisiae is a eukaryotic model organism widely used for the investigation of fundamental cellular processes and disease mechanisms. Consequently, the lipid landscape of yeast has been extensively investigated and up to this day the lipidome is considered as rather basic. Here, we use...
During megakaryopoiesis, megakaryocytes (MK) undergo cellular morphological changes with strong modification of membrane composition and lipid signaling. Here we adopt a lipid-centric multi-omics approach to create a quantitative map of the MK lipidome during maturation and proplatelet formation....
This model is from the article:
Parallel adaptive feedback enhances reliability of the Ca2+ signaling system.
Abell E, Ahrends R, Bandara S, Park BO, Teruel MN. Proc Natl Acad Sci U S A.
2011 Aug 15. 21844332
,
Abstract:
...
This model is from the article:
Parallel adaptive feedback enhances reliability of the Ca2+ signaling system.
Abell E, Ahrends R, Bandara S, Park BO, Teruel MN. Proc Natl Acad Sci U S A.
2011 Aug 15. 21844332
,
Abstract:
...
We introduce here SIMPLEX (SImultaneous Metabolite, Protein, Lipid EXtraction procedure), a unique strategy for the quantitative investigation of lipids, metabolites and proteins. SIMPLEX offers a fundamental turn in study design since multimolecular classes can be accessed in parallel from one samp...
Accumulation of unfolded proteins in the endoplasmic reticulum triggers the unfolded protein response (UPR), an adaptive signal transduction pathway aimed at reinstating cellular homeostasis, or, if that fails, at triggering of apoptosis. The UPR plays a key role in a variety of disorders (including...
Membrane lipids are essential components of synaptic junctions that have key functions in neurotransmission. In the first part of the study, we provide a quantitative lipid inventory of mouse and rat synaptic junctions. We next used a simultaneous multi-omics extraction and analysis workflow termed ...
