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TranscriptomicsRNA-seq analysis of differentiating human erythroblasts
Human erythroblasts purified from cord blood were cultured in vitro and FACS-sorted into five highly purified populations representing distinct differentiation stages:  proerythroblasts, early basophilic erythroblasts, late basophilic erythroblasts, polychromatophilic erythroblasts, and orthochromat...
ORGANISM(S): Homo sapiens 
2013-12-25 | E-GEOD-53635 | biostudies-arrayexpress
TranscriptomicsGlobal Transcriptome Analyses of Mammalian Terminal Erythroid Differentiation
Purpose:The purpose of this study is to create unbiased, stage-specific transcriptomes by RNA-seq analyses of pure populations of both murine and human erythroblasts at distinct developmental stages. Methods: Recently developed FACS-based methods (Chen et al, PNAS, Liu et al, Blood, Hu et al Blood) ...
ORGANISM(S): Mus musculus 
2014-03-19 | E-GEOD-53983 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of whole blood from sickle-cell disease patients using globin mRNA reduction
Room temperature whole blood mRNA stabilization procedures, such as the PAX gene system, are critical for the application of transcriptional analysis to population-based clinical studies. Global transcriptome analysis of whole blood RNA using microarrays has proven to be challenging due to the high ...
ORGANISM(S): Homo sapiens 
2009-06-20 | E-GEOD-16728 | biostudies-arrayexpress
TranscriptomicsIsolation and Transcriptome Analyses of Human Erythroid Progenitors: BFU-E and CFU-E
Burst-forming unit-erythroid (BFU-E) and colony-forming unit-erythroid (CFU-E) cells are erythroid progenitors traditionally defined by colony assays. We developed a flow cytometry-based strategy for isolating human BFU-E and CFU-E cells based on the changes in expression of cell surface markers dur...
ORGANISM(S): Homo sapiens 
2014-10-30 | E-GEOD-61566 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human platelets from sickle cell patients reveals changes in arginine metabolic pathways
In sickle cell disease, ischemia-reperfusion injury and intravascular hemolysis produce endothelial dysfunction and vasculopathy characterized by reduced nitric oxide (NO) and arginine bioavailability. Recent functional studies of platelets in patients with sickle cell disease reveal a basally activ...
ORGANISM(S): Homo sapiens 
2008-06-18 | E-GEOD-11524 | biostudies-arrayexpress
TranscriptomicsLong noncoding RNA expression in several hematopoietic progenitor and differentiated cell populations using a custom Agilent microarray
Mammals express thousands of long noncoding (lnc) RNAs, a few of which are shown to function in tissue development. However, the entire repertoire of lncRNAs and the extent to which they regulate biological processes in different tissues and species are not defined. Indeed, most lncRNAs are not cons...
ORGANISM(S): Mus musculus 
2014-02-20 | E-GEOD-51892 | biostudies-arrayexpress
TranscriptomicsDifferences in gene expression and cytokines levels between newly diagnosed and chronic pediatric immune thrombocytopenia (ITP)
Immune thrombocytopenia (ITP) is an autoimmune disease where platelets are destroyed prematurely. In the majority of children the disease resolves but in some it becomes chronic. To investigate whether the two forms of the disease are similar or separate entities we performed DNA microarray analysis...
ORGANISM(S): Homo sapiens 
2013-08-05 | E-GEOD-46922 | biostudies-arrayexpress
MetabolomicsThe effect of the MYH9 in endothelial cells on metabolism

This document focuses on exploring the specific impact of MYH9 on the metabolic processes of vascular endothelial cells. To achieve this, a well-designed experimental approach was adopted: human umbilical vein endothelial cells (HUVECs) were subjected to different treatments using adenoviruses, i...

2026-03-05 | MTBLS12819 | MetaboLights
TranscriptomicsKnock-out of the nuclear localization in pp65 protein of Cytomegalovirus: biologic and immunologic effects
The CMVpp65 protein contains 2 bipartite nuclear localization signals (NLS) at 415-438aa and 537-561aa near the carboxy terminus of CMVpp65 and a phosphate binding site related to kinase activity at lysine-436. A mutation of pp65 having K436N (CMVpp65mII) and further deletion of aa537-561 resulted ...
ORGANISM(S): Homo sapiens 
2009-01-30 | E-GEOD-14347 | biostudies-arrayexpress
MetabolomicsPrenatal exposure to trace elements impacts mother-infant gut microbiome, metabolome and resistome during the first year of life

Infancy is a critical period for the colonization of the gut microbiome. However, xenobiotic effects on gut microbiome development in early life remain poorly understood. Here, we recruit 146 mother-infant pairs and collect stool samples at 3, 6, and 12 months after delivery for amplicon sequenci...

2025-05-02 | MTBLS11392 | MetaboLights
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