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ProteomicsAnion-Exchange Chromatography of Tryptic and Phosphopeptides: WAX vs. SAX and AEX vs. ERLIC
At a pH > 5, phosphopeptides have two negative charges per residue and are well-retained in anion-exchange chromatography. However, the peptides with one or two phosphate groups are not separated from the peptides with multiple Asp or Glu residues, which interfere with the identification of phosph...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080774 | MassIVE
ProteomicsComparison of Intact Glycopeptide Enrichment Methods Using Strong Anion Exchange and Hydrophilic Interaction Liquid Chromatography
Heterogeneity of protein glycosylation poses great challenge for analysis that is key to un-puzzle systems glycobiology in diseases. Resolving this conundrum requires global enrichment of glycopeptides for identification and quantitation. To this aim, hydrophilic interaction chromatography (HILIC) h...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-07-02 | MSV000082566 | MassIVE
ProteomicsAnion-Exchange Chromatography of Tryptic and Phosphopeptides: WAX vs. SAX and AEX vs. ERLIC
At a pH > 5, phosphopeptides have two negative charges per residue and are well-retained in anion-exchange chromatography. However, the peptides with one or two phosphate groups are not separated from the peptides with multiple Asp or Glu residues, which interfere with the identification of phosph...
ORGANISM(S): Homo sapiens (Human) 
2015-04-23 | PXD001333 | Pride
Chromatography erlic sax wax aex 
ProteomicsDevelopment of sequential sample processing protocol on a single anion-exchange solid-phase extraction disc for bottom-up/cross-linking mass spectrometry
In this project, we developed a rapid enzymatic digestion protocol within an anion exchange StageTip named AnExSP (anion-exchange disc-assisted sequential sample preparation) and, using data-independent acquisition, analyzed its digestion performance of human cellular protein extracts (HCPE) using t...
ORGANISM(S): Homo Sapiens (human) 
2022-04-20 | PXD029444 | JPOST Repository
OtherAnion-Exchange Chromatography of Phosphopeptides: Weak Anion Exchange versus Strong Anion Exchange and Anion-Exchange Chromatography versus Electrostatic Repulsion-Hydrophilic Interaction Chromatography.
Data from ProteomeXchange, PXD ID: PXD001333. File: Amanda ms1-8ppm ms2-20ppm 20130219_QEx3_RSLC-4_Mischerikow_Peters_IMP_shotgun_s2324.msf.mgf. Published as part of Anal Chem. 2015 Apr 17 . From the Abstract: {{i}} ... Weak anion-exchange (WAX) and strong anion-exchange (SAX) columns were compared...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32310019320 | GPMDB
Resource Reanalysis 
ProteomicsSeparation of proteome of Kuenenia stuttgartiensis strain CSTR1 using anion exchange chromatography
We analyzed proteome of the anammox bacterium Kuenenia stuttgartiensis strain CSTR1 after separation by anion exchange chromatography. Each fraction has a volume of 0.2 or 0.4 mL.
ORGANISM(S): Candidatus Kuenenia stuttgartiensis 
2025-02-03 | PXD054997 | Pride
Anion exchange chromatography Anammox Proteome 
ProteomicsAnion Exchange Chromatography-Based Purification of Exosomes from Brassica oleracea L.
A purification platform that integrates ultrafiltration (UF) with anion exchange chromatography in a fast protein liquid chromatography (FPLC) system, which provides superior resolution, reproducibility, and automation, making it an ideal choice for scaling up exosome isolation.
ORGANISM(S): Brassica oleracea (Wild cabbage) 
2025-08-25 | PXD063392 | Pride
Brassica Vescicle Proteins 
TranscriptomicsA user-friendly chromatographic method to purify small regulatory RNAs
The discovery of the small regulatory RNA populations has changed our vision of cellular regulations. Indeed, loaded on Argonaute proteins they formed ribonucleoprotein complexes that target complementary sequences and achieved widespread silencing mechanisms conserved in most eukaryotes. The recent...
ORGANISM(S): Drosophila melanogaster 
2013-06-13 | E-GEOD-40748 | biostudies-arrayexpress
ProteomicsPassive elution of proteoforms from polyacrylamide gels combined with anion exchange purification for in-depth top-down analysis of the human low molecular weight proteome.
We developed a novel sample preparation method by combining Passively Eluting Proteins from Polyacrylamide gels as Intact species for MS (PEPPI-MS) fractionation with Anion-Exchange disc-assisted Sequential sample Preparation (AnExSP) purification, and evaluated its performance in TDP analysis by co...
ORGANISM(S): Homo sapiens (Human) 
2022-09-26 | PXD034353 | Pride
Sds-page Top-down Peppi Anion exchange Stagetip 
UnknownAnion-exchange chromatography of phosphopeptides: weak anion exchange versus strong anion exchange and anion-exchange chromatography versus electrostatic repulsion-hydrophilic interaction chromatography.
Not available
S-EPMC4423237 | biostudies-literature
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