Development of sequential sample processing protocol on a single anion-exchange solid-phase extraction disc for bottom-up/cross-linking mass spectrometry

ABSTRACT: In this project, we developed a rapid enzymatic digestion protocol within an anion exchange StageTip named AnExSP (anion-exchange disc-assisted sequential sample preparation) and, using data-independent acquisition, analyzed its digestion performance of human cellular protein extracts (HCPE) using trypsin and trypsin/Lys-C. The outstanding effectiveness and simplicity of AnExSP in sample preparation for gel-based cross-linking mass spectrometry was also verified.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Nobuaki Takemori 

PROVIDER: PXD029444 | JPOST Repository | Wed Apr 20 00:00:00 BST 2022

REPOSITORIES: jPOST