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MetabolomicsMetabolite assay of T cells that are antigen-specially activated by viscoelastic synthetic antigen presenting cells

Adoptive T cell receptor (TCR)–based immunotherapy offers a powerful strategy to target solid tumors. However, the physical properties of the antigen-presenting interface regulate T cell metabolic programming remains poorly defined. Here we develop synthetic viscoelastic activating cells (SynVAC)...

2026-09-03 | MTBLS15444 | MetaboLights
MetabolomicsPharmacological Targeting of ALDH1a2 in Dendritic Cells Enhances Antigen Presentation, T Cell Activation and Anti-tumor immunity
Dendritic cell (DC) vaccines have been proposed as cancer immunotherapies due to their role as crucial antigen-presenting cells that regulate T cell functions. Despite considerable efforts to optimize ex vivo priming of DCs, DC vaccines have rarely been successful, suggesting the presence of unknown...
2025-11-03 | MTBLS10389 | MetaboLights
MetabolomicsA robust intracellular metabolite extraction protocol for human neutrophil metabolic profiling
BACKGROUND: Neutrophils are phagocytic innate immune cells that play essential roles in host defence, but are also implicated in inflammatory diseases such as rheumatoid arthritis (RA) where they contribute to systemic inflammation and joint damage. Transcriptomic analysis of neutrophils has reveale...
2018-12-06 | MTBLS658 | MetaboLights
MetabolomicsDeep longitudinal multi-omics analysis of Bordetella pertussis cultivated in bioreactors highlights medium starvations and transitory metabolisms, associated to vaccine antigen biosynthesis variations and global virulence regulation

Bordetella pertussis is the bacterial causative agent of whooping cough, a serious respiratory illness. An extensive knowledge on its virulence regulation and metabolism is a key factor to ensure pertussis vaccine manufacturing process robustness. The aim of this study was to refine our ...

2023-02-21 | MTBLS5762 | MetaboLights
ProteomicsSystematic Evaluation and Optimization of Protein Extraction Parameters in Diagnostic FFPE Specimens
Objectives: Formalin-fixed paraffin-embedded (FFPE) tissue is the standard material for di-agnostic pathology but poses relevant hurdles to accurate protein extraction due to cross-linking and chemical alterations. While numerous extraction pro-tocols and chemicals have been described, systematic c...
ORGANISM(S): Homo sapiens (Human) 
2022-05-19 | PXD029133 | Pride
Protein extraction  formalin-fixed paraffin-embedded tissue  clinical prote-omics  antigen retrieval  crosslinking reversal  protein analysis  biomarker 
TranscriptomicsExpression data from antigen-specific CD4+T cells from peripheral blood of patietns with CD and healthy controls.
Crohn's Disease (CD) is a chronic inflammatory disease of the intestinal tract. We performed a whole-genome transcriptional analysis using sorted commensal antigen-specific CD4+T cells from peripheral blood of CD patients and from healthy controls. Peripheral blood sorted FlaX, Fla2 and YidX-specifi...
ORGANISM(S): Homo sapiens 
2016-07-27 | E-GEOD-70469 | biostudies-arrayexpress
ProteomicsGalectin-8 facilitates B cell responses to surface-tethered antigens by promoting antigen extraction at the B cell synapse
Complete activation of B cells relies on their capacity to extract surface-tethered 35  antigens at the immune synapse by either exerting mechanical forces or promoting 36  their proteolytic degradation through lysosome secretion. Whether antigen extraction 37  can be tuned by local cues originating...
ORGANISM(S): Mus musculus (Mouse) 
2018-12-14 | PXD011522 | Pride
B-lymphocytes Processing and presentation Polarity Mouse immunization Immune synapse Antigen extraction Galectin-8 
ProteomicsOptimizing High-Throughput Shotgun Immunoproteomics for Antigen Identification
Identification of individual proteins (i.e., antigens) which initiate and/or perpetuate adaptive immune responses has potential to greatly impact pre-clinical and clinical work across numerous fields. To date, however, the methodologies available to identify antigens responsible for driving adaptive...
ORGANISM(S): Bos taurus (Bovine) 
2023-04-18 | PXD038761 | Pride
Tandem-mass tag labeling Antigen identification Xenogeneic biomaterials Affinity chromatography Humoral immune response Proteomics 
ProteomicsMutations in epitope flanking regions for SARS-CoV-2 nucleoprotein affect proteasomal processing to alter CD8+ T cell responses
Viral CD8+ epitopes are generated by the cellular turnover of viral proteins, predominantly by the proteasome1. Mutations located within viral epitopes can result in escape from memory T cells2. Focussing on two of the most dominant SARS-CoV-2 nucleoprotein CD8+ epitopes3,4, we identified mutations ...
ORGANISM(S): recombinant SARS coronavirus 
2023-10-24 | PXD032054 | Pride
Lc-ms/ms Sars-cov-2 T-cell Antigen Epitope 
TranscriptomicsAntigen specific human conventional T cells (CD154+) or regulatory T cells (CD137+) directed agains A. fumigatus or Birch pollen lysate isolated from Birch pollen allergic patients or healthy controls.
Foxp3+ regulatory T cells (Treg) play a central role for tolerance against self and innocuous environmental antigens. However, the role of antigen-specificity for Treg-mediated tolerance is only incompletely understood. Here we show by direct ex vivo characterization of human CD4+ T cells, that the ...
ORGANISM(S): Homo sapiens 
2016-10-25 | E-GEOD-77018 | biostudies-arrayexpress
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