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ProteomicsCross-linking of mouse laminin 111
Laminin, a ~800 kD heterotrimeric protein, is a major functional component of the extracellular matrix, contributing to tissue development and maintenance. We used cross-linking and mass spectrometry to determine the order of subunits in the ~750 Å trimeric coiled-coil oligomerization domain of lami...
ORGANISM(S): Mus musculus (Mouse) 
2016-12-22 | PXD004898 | Pride
Cross linking Extra cellular matrix Laminin 
ProteomicsThe GlycoPaSER prototype as a real-time N-glycopeptide identification tool based on the PaSER parallel computing platform
Real-time database searching allows for simpler and automated proteomics workflows as it eliminates technical bottlenecks in high throughput experiments. Most importantly, it enables results dependent acquisition (RDA) where search results can be used to guide data acquisition during acquisition. Th...
ORGANISM(S): Homo sapiens (Human) 
2023-03-13 | PXD040716 | Pride
Paser Glycoproteomics Glycopaser Results dependent acquisition (rda) Real-time search 
ProteomicsExtracellular Matrix proteomics
Proteomics of extracellular matrix from fibroblast cell cultures (WI-38). The results of these experiments were used to supprt an electron microscopy study of the extracellular matrix.
ORGANISM(S): Homo sapiens (Human) 
2019-03-13 | PXD007700 | Pride
Extracellular matrix Ecm 
ProteomicsExtracellular whole cell cross-liking of B cells (JY)
We performed XL-MS on the extracellular region of whole B cells (JY). The human leukocyte antigen (HLA) was the most cross-linked membrane protein where the most common interactor of and HLA was another HLA. We were able to model HLA-HLA interaction using the structural information the cross-links p...
ORGANISM(S): Homo sapiens (Human) 
2021-05-28 | PXD022675 | Pride
Xl-ms Human leukocyte antigen (hla) Plasma membrane 
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