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UnknownCoupling bioorthogonal chemistries with artificial metabolism: intracellular biosynthesis of azidohomoalanine and its incorporation into recombinant proteins.
Not available
S-EPMC6271081 | biostudies-literature
UnknownModulation of cellular transcriptome and proteome composition by azidohomoalanine-implications on click chemistry-based secretome analysis.
Not available
S-EPMC10300158 | biostudies-literature
UnknownAn Integrative Biology Approach to Quantify the Biodistribution of Azidohomoalanine In Vivo.
Not available
S-EPMC10121978 | biostudies-literature
UnknownAzidohomoalanine (AHA) Metabolic Labeling Reveals Unique Proteomic Insights into Protein Synthesis and Degradation in Response to Bortezomib Treatment.
Not available
S-EPMC12737224 | biostudies-literature
ProteomicsHEK293T cell translatome analysis upon Torin1 treatment or single amino acid withdrawal. Related to Supplementary Table 6 from An et al (2020)
HEK293T subject to Single AA starvation dataset and translatome analysis was determined using Azidohomoalanine (AHA) incorportation, Biotin-click, and streptavidin enrichment. TMTpro 16plex labels was used.
ORGANISM(S): Homo sapiens (Human) 
2020-04-30 | PXD017861 | Pride
Human Lc-msms Azidohomoalanine (aha) 
ProteomicsAcute synthesis of CPEB is required for plasticity of visual avoidance behavior in Xenopus
Neural plasticity requires protein synthesis, but the identity of newly synthesized proteins generated in response to plasticity-inducing stimuli remains unclear. We used in vivo bio-orthogonal noncanonical amino acid tagging (BONCAT) with the methionine analog azidohomoalanine (AHA) combined with t...
ORGANISM(S): Xenopus Laevis (ncbitaxon:8355) 
2018-01-11 | MSV000081914 | MassIVE
ProteomicsF1 allele specific gene expression
By comparing mouse fibroblasts from two parental strains (Bl6 and Spretus) with fibroblasts from their first generation offspring (F1) we can detect allele specific expression of proteins. The Bl6 and Spretus lines are evolutionary distant and harbour many SNPs in their genomes which when synonomous...
ORGANISM(S): Mus musculus (Mouse) 
2015-08-18 | PXD002337 | Pride
Lc-ms/ms Bl6 Azidohomoalanine Silac F1 Spretus Allele Monolithic column 
ProteomicsHEK293T cell translatome analysis upon 6-MP treatment. Related to Supplementary Table 6 from An et al (2020)
HEK293T cells were treated with 6-MP and harvested at various time point (6, 9 and 18h). Translatome was measure by Azidohomoalanine (AHA) incorporation, Biotin-click, and streptavidin enrichment.
ORGANISM(S): Homo sapiens (Human) 
2020-04-30 | PXD017860 | Pride
Human Lc-msms Azidohomoalanine (aha) incorporation 
ProteomicsQuantification of the translatome in wild type Arabidopsis and amiNAA10 plants
Wild type Arabidopsis Col-0 plants and mutants depleted of the catalytic subunit of the ribosome-associated N-acetyltransferase A (NatA) complex (amiNAA10) were grown on soil for six weeks under short-day conditions (8h light, light intensity: 100 µE, 21 °C/18 °C temperature (day/night), 50 % humidi...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-04-04 | PXD024328 | Pride
Arabidopsis thaliana Co-translational protein modification N-acetyltransferase a N-terminal protein acetylation Translation 
ProteomicsHEK293T cell degradome (pulse chase) analysis upon 12h Torin1 treatment. Related to Supplementary Table 3 from An et al (2020)
HEK293T Azidohomoalanine (AHA) Pulse Chase (degradome) experiment, were cells from various genetic background (WT, ATG7-/-, RB1CC1-/-) are treated or not with Torin1 for 12h. Samples are Biotin-click, and streptavidin enriched.
ORGANISM(S): Homo sapiens (Human) 
2020-04-30 | PXD017857 | Pride
Human Lc-msms Azidohomoalanine (aha) incorporation 
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