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ProteomicsHEK293T cell translatome analysis upon 6-MP treatment. Related to Supplementary Table 6 from An et al (2020)
HEK293T cells were treated with 6-MP and harvested at various time point (6, 9 and 18h). Translatome was measure by Azidohomoalanine (AHA) incorporation, Biotin-click, and streptavidin enrichment.
ORGANISM(S): Homo sapiens (Human) 
2020-04-30 | PXD017860 | Pride
Human Lc-msms Azidohomoalanine (aha) incorporation 
ProteomicsHEK293T cell degradome (pulse chase) analysis upon 12h Torin1 treatment. Related to Supplementary Table 3 from An et al (2020)
HEK293T Azidohomoalanine (AHA) Pulse Chase (degradome) experiment, were cells from various genetic background (WT, ATG7-/-, RB1CC1-/-) are treated or not with Torin1 for 12h. Samples are Biotin-click, and streptavidin enriched.
ORGANISM(S): Homo sapiens (Human) 
2020-04-30 | PXD017857 | Pride
Human Lc-msms Azidohomoalanine (aha) incorporation 
ProteomicsOptimization of protocols for detection of de novo protein synthesis in whole blood samples via azide-alkyne cycloaddition
Aberrant protein synthesis and protein expression are a hallmark of many disorders ranging from cancer Aberrant protein synthesis and protein expression are a hallmark of many disorders ranging from cancer to neuropsychiatry. Blood-based biomarkers indicative of changes in proteomes have long been h...
ORGANISM(S): Rattus norvegicus (Rat) 
2020-10-08 | PXD019243 | Pride
De novo protein synthesis Blood-based biomarkers Copper(i)-catalyzed click-chemistry Whole blood Ethylenediaminetetraacetic acid 
ProteomicsBONCAT in Arabidopsis cell culture LC-MS
Determining which proteins are actively synthesised at a given point in time and being able to extract them for analysis is important to understanding plant responses to environmental or developmental stimuli, but there are few verified experimental tools to do this directly or quantitatively. In-vi...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-06-23 | PXD023170 | Pride
Boncat Arabidopsis Lc-ms Cell culture Noncanoical amino acids 
UnknownAn Integrative Biology Approach to Quantify the Biodistribution of Azidohomoalanine In Vivo.
Not available
S-EPMC10121978 | biostudies-literature
UnknownPhoto-Methionine, Azidohomoalanine and Homopropargylglycine Are Incorporated into Newly Synthesized Proteins at Different Rates and Differentially Affect the Growth and Protein Expression Levels of Auxotrophic and Prototrophic E. coli in Minimal Medium.
Not available
S-EPMC10380393 | biostudies-literature
UnknownLipidated variants of the antimicrobial peptide nisin produced via incorporation of methionine analogs for click chemistry show improved bioactivity.
Not available
S-EPMC10404616 | biostudies-literature
UnknownDual-Functional-Tag-Facilitated Protein Labeling and Immobilization.
Not available
S-EPMC6044709 | biostudies-literature
UnknownExploring structural dynamics of a membrane protein by combining bioorthogonal chemistry and cysteine mutagenesis.
Not available
S-EPMC6850778 | biostudies-literature
ProteomicsOptimization of Bio-Orthogonal Non-Canonical Amino acid Tagging (BONCAT) for effective low-disruption labelling of Arabidopsis proteins in vivo
Plants, as sessile organisms, require robust responses in protein synthesis to adapt to variable environmental conditions. Measurement of newly synthesized proteins has been successfully facilitated with Bio-Orthogonal Non-Canonical Amino acid Tagging (BONCAT) across a multitude of organisms. Here, ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-06-08 | PXD064210 | Pride
Mass spectrometry Boncat Click chemistry Quantitative proteomics 
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